| 唐浏英,邱海燕,李元凯,张伟,尚德秋,胡德育,张福利,刘冬立,李巧林,王进,李秋芬.多聚酶链反应用于布鲁氏菌病病人诊断的流行病学评价[J].Chinese journal of Epidemiology,1997,18(3):153-155 |
| 多聚酶链反应用于布鲁氏菌病病人诊断的流行病学评价 |
| The Epidemiologic Value of Polymerase Chain Reaction to the Diagnosis of Brucellosis Patients |
| Received:November 01, 1996 Revised:November 07, 1996 |
| DOI： |
| KeyWord: 布鲁氏菌病 分子流行病学 PCR |
| English Key Word: Brucellasis Molecular Epidemiology PCR |
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| Abstract: |
| 我们首次用分子生物学方法（PCR）对布病暴发流行的疫区-陕西省绥德县义合乡和满堂川乡54例布病病人和36例对照进行了研究，发现以SAT1：100++为诊断标准诊断的布病病人中，PCR的阳性率为75%，而生活环境相同的以相同的标准判定的对照人群PCR的阳性率是56.7%，二者差异显著，P<0.05。两组人群RBPT的阳性率分别是96.3%和30.6%。同时，我们还检查了免疫组8人，免疫时间从一个月到32年不等，PCR和RBPT的阳性率分别是100%和87.5%。对急性、亚急性和慢性期布病病人PCR的阳性率进行统计学处理，结果P>0.10，差异不显著。这表明：以查抗体为主的血清学结果与感染或免疫的时间长短有关，而检查靶DNA的PCR方法则不受感染病期或免疫时间的影响。 |
| English Abstract: |
| PCR was used to test fifty-four brucellasis patients and thirty-six healthy people as controls, who were all from Man Tang Chuan and Yi He communities, Sui De county, Shan Xi Province,where brucellosis out-break was occurred in 1996. The diagnostic titres of SAT for brucellasis patients was set 1:100++. The positive rates of PCR in patients and controls were 75%and 65.7% respectively,comparing with the positive rates of RBPT 96.3% and 30.6% in the same two groups. At the same time, we also detected eight people who had received immunization one month to thirty-two years ago,with positive rates for PCR and RBPT 100% and 87.5% respectively. The results showed that there was no significant diversity of PCR results among acute,subacute and chronic brucellosis patients. It was revealed that the serological results were related to the course of disease,but the results came out of PCR method which was used to detect target DNA was not influenced by the course of disease. |
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