| 刘明团,王树声,朱田风,韦一知.PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸[J].Chinese journal of Epidemiology,1997,18(3):164-166 |
| PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸 |
| Detection of Poliovirus by Digoxigenin-labeled cDNA Probe Prepared by PCR Technique |
| Received:January 06, 1997 Revised:January 16, 1997 |
| DOI： |
| KeyWord: PCR 地高辛标记 核酸斑点杂交 |
| English Key Word: PCR Digoxigenin-labeling Dot hybridization |
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| Abstract: |
| 笔者应用聚合酶链反应（PCR）合成系统，以逆转录的SabinⅠ、Ⅱ、Ⅲ型病毒cDNA为模板，在反应液中加入标记的Dig-dUTP，经扩增制备了地高辛配基标记的脊髓灰质炎病毒cDNA探针、该法比PCR扩增产物后，电泳，片段回收到标记、提纯，常需3天。结果比较PCR技术直接制备地高辛标记cDNA探针方便，快速，标记率高，用于型别鉴定比中和试验快，敏感，特异性强等优点。 |
| English Abstract: |
| A study including reverse transcribed cDNAs of Sabin Ⅰ, Ⅱ, Ⅲ virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid,more sensitive and more specific for poliovirus typing. |
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