北京郊区旅游景点北亚蜱传斑点热分子流行病学调查

陈敏; 范明远; 毕德增

Abstract

陈敏,范明远,毕德增.北京郊区旅游景点北亚蜱传斑点热分子流行病学调查[J].Chinese journal of Epidemiology,1997,18(4):197-200

北京郊区旅游景点北亚蜱传斑点热分子流行病学调查

A Molecular Epidemiologic Investigation of North Asia Fever in Scenic Spots of Beijing Suburb

Received:March 21, 1997 Revised:April 08, 1997

DOI：

KeyWord: 北亚蜱传斑点热分子流行病学

English Key Word: North Asia fever Molecular epidemiology

FundProject:本课题为国家自然科学基金资助项目

Author Name	Affiliation
Chen Min	Department of Rickettsiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Fan Ming-yuan	Department of Rickettsiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206
Bi De-zeng	Department of Rickettsiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206

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Abstract:

用PCR/RFLP技术对北京郊区11个旅游景点的媒介蜱及小哺乳动物进行斑点热群立克次体的检测，并进行了病原体分离和鉴定。结果从北京航空博物馆附近地区收集的刺猬和其体表寄生的中华革蜱中分离出2株斑点热群立克次体，分别命名为BJH-95株和BJ-95株，经SDS-PAGE、Western blot和PCR/RFLP技术进行了鉴定认为是西伯利亚立克次体。结果证明了该立克次体在蜱和动物宿主间呈水平传播，并且表明在被调查的旅游景点中除航空博物馆附近地区外，大多数景点基本上是北亚热的安全区。在刺猬中分离出西伯利亚立克次体为国内、外首次报道。

English Abstract:

PCR/RFLP technique was used to detect spotted fever group rickettsiae (SFGR) in ticks and small mammals collected in eleven scenic spots of Beijing suburb.We not only detected Rickettsia sibirica in D.sinicus and hedgehog collected nearby the Museum of Aviation, but also isolated two strains of SFGR from them,named as BJ-95 strain and BJH-95 strain respectively. The two strains were identified as R.sibirica by SDS-PAGE,Western blot and PCR/RFLP. The results demonstrated the existence of horizontal transmission of R.sibirica between ticks and small mammals and showed the most scenic spots except the vicinity of Museum of Aviation being investigated were safe to North Asia Fever.This is the first report on the isolation of R.sibirica in hedgehogs.

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