| 刘红梅,郭存三,魏文杰,董明.对我国部分Ⅰ型脊髓灰质炎病毒的分子流行病学分析[J].Chinese journal of Epidemiology,1997,18(4):208-210 |
| 对我国部分Ⅰ型脊髓灰质炎病毒的分子流行病学分析 |
| Studies on the Molecular Epidemiology of Poliovirus Type 1 in China |
| Received:February 24, 1997 Revised:March 21, 1997 |
| DOI： |
| KeyWord: 脊髓灰质炎病毒 聚合酶链式反应 点杂交 DNA序列测定 |
| English Key Word: Poliovirus PCR Dot blot hybridization DNA sequencing |
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| Abstract: |
| 作者用PCR、点杂交方法和核苷酸序列分析验证了1988年流行于山东和1990年流行于新疆的Ⅰ型脊灰病毒为野毒株，它们VP1-2A区150个核苷酸序列与SabinⅠ疫苗株的差异率≥ 16.7%。而3株新疆株之间在此区域内的核苷酸差异率≤ 2.67%，编码的氨基酸序列完全相同。山东株在此区域与新疆株核苷酸序列的差异率达14.0%~15.33%，并且有一个氨基酸不同。提示山东株与新疆株属不同的野毒基因型。 |
| English Abstract: |
| A strain of poliovirus type 1 isolated from an endemic in Shandong Province in 1988 and three strains of poliovirus type 1 isolated from an endemic in Xinjiang in 1990 were analysed by dot blot hybridization with Sabin Ⅰ specific probe and PCR amplification with Sabin Ⅰspecific primers. They were proved to be non Sabin like poliovirus. The nucleotide sequences of VP1-2A junction region of four poliovirus type 1 isolates were analysed. It was found that the nucleotide diversity of four isolates with Sabin Ⅰ was equal to or greater than 16.7%.This finding proved that they were wild polioviruses and greatly different fromSabin Ⅰ.It was also found that the nucleotide sequence diversity among three strains from Xinjiang was very small (less than 2.6%) with identical amino acid sequences. This indicated that the three Xinjiang strains belonged to the same genotype. While the difference of Shandong strain from the three Xinjiang strains was between 14.0% to 15.33% in nucleotide sequence and 2.0% to 4.0% in amino acids. This indicated that Shandong strain was apparently different from three Xinjiang strains and it belonged to another wild genotype which had a different history of evolution. |
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