| 陈晶晶,张建中,蒋秀高,杨昭徐,梁丕霞.不同人群幽门螺杆菌表型分型研究[J].Chinese journal of Epidemiology,1997,18(6):338-340 |
| 不同人群幽门螺杆菌表型分型研究 |
| Typing of H. pylori Strains Isolated from Patients with Gastric Diseases |
| Received:July 09, 1997 |
| DOI： |
| KeyWord: 幽门螺杆菌 菌型 SDS-PAGE分析 |
| English Key Word: Helicobacter pylori Typing SDS-PAGE |
| FundProject:本课题受国家自然科学基金资助 |
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| Abstract: |
| 作者报道了从各型胃病患者胃粘膜中分离的112株幽门螺杆菌，用聚丙烯酰胺凝胶电泳（SDS-PAGE）及Western blots分析，可将HP菌分为Ⅰ-Ⅳ型，而其中Ⅳ型又分为Ⅳ-1至Ⅳ-5五个亚型；不同HP间存在明显的蛋白抗原差异和LPS/LOS的表达差异；为今后开展HP疫苗研究提供了重要线索。 |
| English Abstract: |
| Helicobacter pylori, an organism recently described in association with gastritis and peptic lesions. The degree of relationship between these micro-organisms was studied on molecular level usingSDS-PAGE protein profiles and immunoblot patterns. Study on a total of 112 lysates of H. pylori showed some differences in protein patterns of different H. pylori strains. However, the difference became more apparent when studied by immunoblot analysis. According to SDS-PAGE protein, strains isolated from gastric mucous of the patients were identified as types Ⅰ-Ⅳ, while type Ⅳ can be further classified as sub-type Ⅳ-Ⅰto Ⅳ-5 analysis of SDS-PAGE protein patterns proved to be an excellent method of characterizing those H. pylori, different from traditional methods. |
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