PCR方法捕获环境水样中嗜肺军团菌DNA

卢锡华; 万超群; 任红宇; 宫仕梅; 王琳

Abstract

卢锡华,万超群,任红宇,宫仕梅,王琳.PCR方法捕获环境水样中嗜肺军团菌DNA[J].Chinese journal of Epidemiology,1997,18(6):345-347

PCR方法捕获环境水样中嗜肺军团菌DNA

The Detection of Legionella Pneumophila DNA with Polymerase Chain Reaction in Water Samples

Received:July 09, 1997 Revised:August 12, 1997

DOI：

KeyWord: 嗜肺军团菌多聚酶链反应

English Key Word: Legionella pneumophila Ploymerase chain reaction

FundProject:

Author Name	Affiliation
Lu Xi-hua	Institute of Epidemiology and microbiology Chinese Academy of Preventive Medicine, Beijing 102206
Wang Lin	中国医科大学呼吸疾病研究所
Wan Chao-qun	Institute of Epidemiology and microbiology Chinese Academy of Preventive Medicine, Beijing 102206
宫仕梅	Institute of Epidemiology and microbiology Chinese Academy of Preventive Medicine, Beijing 102206
王琳	Institute of Epidemiology and microbiology Chinese Academy of Preventive Medicine, Beijing 102206

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Abstract:

嗜肺军团菌是军团病的主要病原体，它所导致的军团病病例占临床病例的80%以上，多数军团病暴发由嗜肺军团菌所引起。通过针对嗜肺军团菌种保守基因（mip gene）设计的引物，我们建立了一种多聚酶链反应（PCR）检测方法，可以准确捕获环境标本中的嗜肺军团菌，同时具有很好的特异性，其最低可检出18cfu/ml的细菌。该方法的建立为嗜肺军团菌病的诊断以及在军团病流行病学调查中嗜肺军团菌病原的追踪提供了一条便捷、准确、可靠的途径。

English Abstract:

Legionella pneumophila has been known as an important pathogen causing Legionnaires' Disease (LD), responsible for more than 80 per cent of clinical LD cases and majority of the LD outbreaks. In order to detect the existence of Legionella pneumophila strains in water samples, we have developed a ploymerase chain reaction method, using two primers synthesized according to the reported Legionella pneumophila mip gene nucleotide sequence. This method was proved to be highly sensitive(18 cfu/ml) and specific. Two water samples were detected positive. This newly developed method provided a quick, accurate way not only to detect Legionella pneumophila in the diagnosis of LD but also in the capture of suspected pathogen during epidemiologic investgation when LD outbreaks.

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