四川省HIV- 1 流行毒株的基因序列测定和亚型分析

秦光明; 邵一鸣; 刘刚; 苏玲; 张灵麟; 管永军; 郑国英; 陈钧; 马义才; 赵全壁; Josef Kostler; Hans Wolf

Abstract

秦光明,邵一鸣,刘刚,苏玲,张灵麟,管永军,郑国英,陈钧,马义才,赵全壁,Josef Kostler,Hans Wolf.四川省HIV- 1 流行毒株的基因序列测定和亚型分析[J].Chinese journal of Epidemiology,1998,19(1):39-42

四川省HIV- 1 流行毒株的基因序列测定和亚型分析

Subtype and Sequence Analysis of the C2- V3 Region of gp120 Genes among HIV-1 Strains in Sichuan Province

DOI：

KeyWord: 人免疫缺陷病毒I 型亚型基因变异

English Key Word: HIV- 1 Subtype Gene variation

FundProject:

Author Name	Affiliation
Qin Guangming	Sichuan Sanitation and Anti-epidemic Station, Chengdu 610031
Shao Yiming	中国预防医学科学院艾滋病参比实验室
Liu Gang	Sichuan Sanitation and Anti-epidemic Station, Chengdu 610031
苏玲	中国预防医学科学院艾滋病参比实验室
张灵麟	Sichuan Sanitation and Anti-epidemic Station, Chengdu 610031
管永军	中国预防医学科学院艾滋病参比实验室
郑国英	Sichuan Sanitation and Anti-epidemic Station, Chengdu 610031
陈钧	中国预防医学科学院艾滋病参比实验室
马义才	Sichuan Sanitation and Anti-epidemic Station, Chengdu 610031
赵全壁	中国预防医学科学院艾滋病参比实验室
Josef Kostler	德国累根斯堡大学医学微生物学研究所
Hans Wolf	德国累根斯堡大学医学微生物学研究所

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Abstract:

使用PCR 对9 份采集于1996 年中旬的四川省经血液途径感染的HIV- 1 阳性感染者外周血单个核细胞( PBMCs) 样品进行扩增, 获得H IV- 1 膜蛋白( env) 基因的核酸片段, 并对其C2- V3 及邻区350~ 450 个核苷酸序列进行了测定和分析。结果表明, 9 份样品中存在B 和C 两种亚型的H IV- 1 毒株序列, 其中B 亚型5 份, 彼此间的基因离散率为1. 4%; C 亚型4 份, 在不包括sc3 号样品的情况下彼此间的基因离散率为2. 6%, 而sc3 号样品与其它3 份样品在核苷酸序列上的差异却达11. 5%。与A~ E 参考亚型及部分B 和C 亚型代表株序列相比较, 属四川B 亚型的5个毒株与包括泰国、缅甸及中国云南德宏在内的B 亚型毒株序列十分接近, 基因离散率在3. 6%~4. 3%的范围内; 属四川C 亚型的4 个毒株除sc3 外则与代表印度毒株的C 亚型共享序列及德宏C亚型毒株序列十分相似, 其基因离散率均为3. 1%, 与非洲C 亚型毒株nof 的基因离散率为13. 1%, 而sc3 号序列与印度、中国德宏和非洲C 亚型HIV- 1 的基因离散率却在10. 1%~ 17. 2%的范围内。提示, HIV- 1 在四川的流行时间不长, 且其B 和除sc3 之外的C 亚型毒株的传入与流行在云南德宏州的相同亚型H IV- 1 毒株密切相关, 而由sc3 所代表的C 亚型毒株则存在目前还尚不了解的其它传入来源。

English Abstract:

Nine DNA fragments of HIV- 1 envgene were amplified by PCR from uncultured peripheral blood mononuclear cells ( PBMCs) obtained from HIV- 1 sero- positive intravenous drugusers ( IDUs) in Sichuan province, in mid 1996. Their C2-V3 region ( about 450 bp) was sequenced. Sequence analysis showed that there were two HIV- 1 subtypes B and C, with 1. 4% and 2. 6% gene divergence inside each subtype, in Sichuan. The 5 subtype B strains, when comparing with sequences of several reference HIV - 1 strains, were found similar to those identified in Thailand, Myanma and Yunnan province. The nucleotide sequence divergence between them was ranged from 3. 6% to 4. 3%. Mean while, the 4 subtype C strains except sc3, were most close to those found in I ndia as well as in Yunnan province, and both their nucleotide sequence diverg ence was 3. 1%. The sc3 strain belonged to subtype C but had larger genetic distance, between 10. 1%- 17. 2%. Above results suggested a recent epidemic of both subtype HIV- 1 strains in Sichuan provine and they were very close to those identified in Yunnan province. The subtype C strain of sc3 is special and its source remains notclear.

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