| 詹思延,高原原,尹香君,黄玉婵,胡永华,李立明.同型半胱氨酸代谢异常与原发性高血压的病例对照研究[J].Chinese journal of Epidemiology,2000,21(3):194-197 |
| 同型半胱氨酸代谢异常与原发性高血压的病例对照研究 |
| A case-control study on the relationship between abnormal homocysteine metabolism and essential hypertension |
| Received:January 25, 2000 |
| DOI： |
| KeyWord: 同型半胱氨酸 亚甲基四氢叶酸还原酶基因 原发性高血压 病例对照研究 |
| English Key Word: Homocysteine MTHFR Hy pertension Case-control study |
| FundProject:教育部博士点基金资助（编号：9723） |
| Author Name | Affiliation | | ZHAN Siyan | Department of Epidemiology, Beijing Medical University, Beijing 100083, China | | GAO Yuanyuan | Department of Epidemiology, Beijing Medical University, Beijing 100083, China | | YIN Xiangjun | Department of Epidemiology, Beijing Medical University, Beijing 100083, China | | 黄玉婵 | Department of Epidemiology, Beijing Medical University, Beijing 100083, China | | 胡永华 | Department of Epidemiology, Beijing Medical University, Beijing 100083, China | | 李立明 | 上海市第二人民医院检验科 |
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| Abstract: |
| 目的 探讨同型半胱氨酸代谢异常与原发性高血压的关系。方法 从上海市一个社区中随机选取127例 35~75岁的原发性高血压病人和 170例正常血压者。使用高效液相色谱结合电化学方法检测血清中同型半胱氨酸总浓度，使用放射免疫法同时测定血清中叶酸和B12 浓度。采用聚合酶链反应 限制性片段长度多态性分析亚甲基四氢叶酸还原酶（MTHFR）基因多态性。结果 调整年龄和性别后，病例和对照组同型半胱氨酸水平分别为10.56μmol/L和10.34μmol/L，差异无显著性（F =0.234，P =0.63）。在未服降压药的对象中同型半胱氨酸浓度与收缩压和舒张压亦无关联。该人群MTHFR不耐热性基因突变频率为 13.1%，突变等位基因频率为 38.7%。病例组与对照组基因型分布和突变等位基因频率差异无显著性。然而，病例组叶酸和B12 浓度高于对照组。结论 本研究未发现升高的同型半胱氨酸水平、MTHFR基因突变是原发性高血压的独立危险因素。高血压病人较高的叶酸和B12 水平可能降低了同型半胱氨酸的危险性。 |
| English Abstract: |
| Objective To examine the relationship between abnormal homocysteine me tabolism and essential hy pertension in a Chinese popula tio n, a community-based case-control study w as conducted.Methods 127 essential hy pertensive patients aged 35 to 75 were randomly selected from a community. Another 170 control subjects with blood pressure<140/90 mmHg were selected from the same
community.Serum homocy steine w as determined using HPLC.Folate and vitamin B12 were measured by radioimmunoassay.MTHFR g enotypes were identified by PCR and restriction fragment length polymorphism analy sis with Hinf Ⅰ digestion.Results After adjusting for age and sex, the mean homocy steine level was 10.56 μmol/L for hypertensive patients and 10.34 μmol/L fo r co ntrols（F =
0.234, P =0.63).No association between either SBP or DBP and Hcy co ncentration w as found in subjects without anti-hy pertensive medications.The prevalence of homo zygousity for thermo labile M THFR variant for this population was 13.1%, and the mutant allele frequency w as 38.7%.There w as no sig nificant difference on genotype distributions and the mutant allele frequency fo r the tw o studied g roups.However, the concentrations of folate and B12 for the hypertensive subjects were g enerally higher than the controls. Conclusions The present study did no t disco ver that the elevation of Hcy levels o r M THFR mutation were independent risk factors fo r essential hyper tension.The hig her folate and B12 in the hypertensiv e subjects might contribute to a lower risk. |
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