哈尔滨市儿童Ⅰ型糖尿病与HLA-DRB1等位基因的关联研究

张慧颖; 王滨有; 赵雪妮; 孙俭宏; 梁子君; 张冬梅; 杨泽; 孙逸平; 沈晶晶

Abstract

张慧颖,王滨有,赵雪妮,孙俭宏,梁子君,张冬梅,杨泽,孙逸平,沈晶晶.哈尔滨市儿童Ⅰ型糖尿病与HLA-DRB1等位基因的关联研究[J].Chinese journal of Epidemiology,2000,21(4):267-269

哈尔滨市儿童Ⅰ型糖尿病与HLA-DRB1等位基因的关联研究

The susceptible alleles on HLA-DRB1 of type Ⅰ diabetes in children in Harbin

Received:October 21, 1999

DOI：

KeyWord: 糖尿病,儿童Ⅰ型(IDDM) 人类白细胞抗原DR等位基因 IDDM易感/保护基因

English Key Word: Type Ⅰ diabetes mellitus (IDDM) HLA-DR IDDM susceptibility / protection genes

FundProject:

Author Name	Affiliation
ZHANG Huiying	Department of Epidemiology, Harbin Medical University, Harbin 150001, China
WANG Binyou	Department of Epidemiology, Harbin Medical University, Harbin 150001, China
ZHAO Xueni	黑龙江省医院儿科
Sun Jianhong	哈尔滨医科大学第二附属医院
Liang Zijun	哈尔滨医科大学第一附属医院
Zhang Dongmei	哈尔滨市儿童医院儿科
Yang Ze	卫生部北京老年医学研究所遗传室
Sun Yiping	卫生部北京老年医学研究所遗传室
Sheng Jingjing	卫生部北京老年医学研究所遗传室

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Abstract:

目的哈尔滨市儿童Ⅰ型糖尿病与人类白细胞抗原DR位点(HLA-DRB1)等位基因的关联关系。方法选取47例0～14岁发病的患者和94名非Ⅰ型糖尿病健康儿童对照，运用PCR-SSP技术测定HLA-DRB1等位基因型别。结果在DR位点上，病例组的DRB1.0301频率和DR3/4杂合子频率显著高于非Ⅰ型糖尿病对照组，而DR2的频率显著低于对照组；DR4亚型中的DRB1.0405频率为病例组高于对照组，RB1.0406频率低于对照组；DRB1.0901频率在两组之间差异无显著性。结论 DR位点的DRB1.0301等位基因为哈尔滨市儿童Ⅰ型糖尿病的强易感基因，而DR2为保护性基因。DR3/4杂合子为易感性杂合子。证实了DR4亚型具有双重作用。未证实DRB1.0901有强易感性，因此，其在中国人Ⅰ型糖尿病患者的易感性作用还有待于进一步探索。

English Abstract:

Objective To evaluate association between type Ⅰ diabetes in children and HLA-DRB1 alleles in Harbin. Methods Case-control method was employed, and the PCR-SSP technique was used to identify HLA-DRB1 alleles. Results The frequencies of DRB1*0301 and DR3/4 heterozygosity were significantly higher but the frequency of DR2 was significantly lower in cases than that in controls. In DR4 subtypes, the frequency of DRB1*0405 was higher and the frequency of DRB1*0406 was lower in cases than that in the controls. The difference between cases and controls in DRB1*0901 was not significant. Conclusion DRB1*0301 allele and DR3/4 heterozygosity were the important susceptibility and heterozygosity genes while DR2 was the most important protective gene in Harbin. The DR4 possessed double-tendency. DRB1*0405 confered susceptibility and DRB1*0406 confered little protection. The susceptibility conferred by DRB1*0901 was not certain in type Ⅰ diabetes in childhood in China. This study provided useful genetic data on the study of etiology of type Ⅰ diabetes mellitus.

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