中国蒙古族人群中人类免疫缺陷病毒1感染相关的基因多态性的初步研究

杜清友; 王福生; 洪卫国; 刘明旭; 金磊; 施红; 雷周云; 额尔敦

Abstract

杜清友,王福生,洪卫国,刘明旭,金磊,施红,雷周云,额尔敦.中国蒙古族人群中人类免疫缺陷病毒1感染相关的基因多态性的初步研究[J].Chinese journal of Epidemiology,2000,21(6):413-416

中国蒙古族人群中人类免疫缺陷病毒1感染相关的基因多态性的初步研究

Polymorphisms of chemokine receptor alleles influencing genetic susceptibility to HIV-1 infection in Mongolia population in China

Received:July 26, 2000

DOI：

KeyWord: HIV-1协同受体基因频率抗性基因

English Key Word: HIV-1 coreceptor Allele frequency Genetic resistance

FundProject:国家自然科学基金资助项目(397706830 )

Author Name	Affiliation	E-mail
DU Qingyou	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China
WANG Fusheng	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China	fswang@public.bta.net.cn
HONG Weiguo	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China
LIU Xuming	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China
JIN Lei	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China
SHI Hong	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China
LEI Zhouyun	Division of Bioengineering, the 302nd Hospital of PLA, Beijing 100039, China
E Erdun	内蒙古呼和浩特市解放军第二五三医院免疫研究室

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Abstract:

目的　调查中国蒙古族人群中与人类免疫缺陷病毒 1(HIV 1)感染相关的等位基因CCR5 Δ32、CCR2b 6 4I和SDF1 3′A的频率和多态性分布。方法　随机采集血样,提取基因组DNA,经聚合酶链式反应 (PCR)或聚合酶链式反应限制性片段长度多态性 (PCR RFLP)电泳分析,计算各个突变基因的频率；并对其群体分布、性别分布和三者的相关性进行统计分析,同时与其他已报道的种族人群进行比较。结果等位基因的突变频率分别为CCR5 Δ32 1.1%、CCR2b64I24.8%、SDF13′A 22.0 %。三种突变等位基因群体分布均符合Hardy Weinberg平衡；CCR2b 6 4I突变频率在性别上的分布有明显差异 (P<0.1),女性的分布频率高于男性,而其他两个等位基因在性别之间差异无显著性；任何两个基因之间也未发现有相关性。结论同美国白种人群相比,中国蒙古族CCR2b 6 4I和SDF1 3′A等位基因的突变频率比较高,与汉族人群的接近,而CCR5 Δ32突变频率比美国白人低,而比汉族人群的高,有关机制有待于进一步阐明。

English Abstract:

Objective Mutant frequency and polymo rphism of HIV-1 resistance CCR5-Δ32、CCR2b-64I and SDF1-3′A alleles were investigated in Chinese population from Mongolian ethnic origin.Methods　Whole blood samples from 134 Mongolian subjects were collected randomly and their g enomic DNA were ex tracted using Qiagen Blood Kit.Allelic frequency was identified by means of PCR or PCRRFLPanalysis.Allelic polymo rphism in population and betw een sex in the sample as well as corelation of the three genes w ere analyzed by χ² test.Results The frequencies of the three alleles were as following :CCR5-Δ32 1.1 %, CCR2b-64I 24.8 % and SDF1-3′A 22.0% respectively.Distribution of the three mutant alleles among the Mongolian populatio n was in accordance with Hardy-Weinberg equilibrium. Statistical analy sis show ed there w as a higher frequency o f CCR2b-64I in female than in male subjects(29.2 % vs 19.7 %).No Statistical difference w as found in the allelic frequencies of bo th CCR5-Δ32 and SDF1-3′A betw een male and female individuals.Conclusion Compared w ith the Caucasian American,there were higher frequencies of CCR2b-64I and SDF1-3′A alleles and low er frequency of CCR5-Δ32 allele found in Mongolian population while the factors responsible for the variation of genetic polymo rphisms in different ethnic populations need to be clearified.

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