| 高哲平,尹炜玲,韩呈武,张京,靳维声,李雪清.氧化电位水对HBsAg破坏效果的试验及临床观察[J].Chinese journal of Epidemiology,2001,22(1):40-42 |
| 氧化电位水对HBsAg破坏效果的试验及临床观察 |
| Observation on the effect of disinfection to HBsAg by electrolyzed oxidizing water |
| Received:May 12, 2000 |
| DOI： |
| KeyWord: 氧化电位水 消毒 乙型肝炎表面抗原 |
| English Key Word: Electroly zedoxidizing water(EOW) Disinfection HBsAg |
| FundProject: |
| Author Name | Affiliation | | GAO Zheping | Nosocomial In fection Department China-Japan Friendship Hospital, Beijing 100029, China | | YIN Weiling | Nosocomial In fection Department China-Japan Friendship Hospital, Beijing 100029, China | | HAN Chengwu | Nosocomial In fection Department China-Japan Friendship Hospital, Beijing 100029, China | | ZHANG Jing | Nosocomial In fection Department China-Japan Friendship Hospital, Beijing 100029, China | | JING Weisheng | Nosocomial In fection Department China-Japan Friendship Hospital, Beijing 100029, China | | LI Xueqing | Nosocomial In fection Department China-Japan Friendship Hospital, Beijing 100029, China |
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| Abstract: |
| 目的: 观察氧化电位水 (EOW)在胃镜消毒中对乙型肝炎表面抗原 (HBsAg)抗原性的破坏效果。方法: 模拟现场试验用EOW与人工污染的胃液作用 1min，观察其对HBsAg的破坏作用，以PBS作为对照组。现场试验以HBsAg阳性病人用后的污染胃镜，作为消毒前对照，经去有机物处理及EOW浸泡 1min，胃镜表面涂抹采样，用ELISA检测HBsAg。结果: 人工污染胃液 (HBsAg平均S/N值为 42.16 )加入胃液量 100倍的EOW作用 1min后，HBsAg检测为阴性，对照组仍为阳性。临床污染胃镜 (HBsAg平均S/N值为5.99)经清洁、消毒后HBsAg检测为阴性。 结论: 在清除有机物后，氧化电位水可有效的破坏污染胃镜HBsAg的抗原性。 |
| English Abstract: |
| Objective: Observation on the effect of disinfection on gastroscope, contaminated by hepatitis B surface antigen (HBsAg)in the electroly zed oxidizing water(EOW). Methods: Contaminated gastric juice and serum was added to EOW for 1 minute. Positive control samples were treated with PBS instead of EOW in the same way. Gastroscopes used for hepatitis patients were immersed in the EOW for 1 minute after cleaning. Samples were collected before and after treatment. ELISA was used to test HBsAg. Results: With mixed samples (average S/N =42.16)of EOW, HBsAg became negative when diluted in 100 times. However, the HBsAg of positive control samples remained positive. After cleaning thegastroscope (average S/ N =5.99)immersed in EOW, HBsAg became negative. Conclusion: EOW was effective in destroy ing HBsAg which could be used for gastroscope disinfection. |
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