我国部分地区丙型肝炎病毒基因分型研究

庄辉; TracyL; 崔怡辉; 凌斌华; 张玲霞; BowdenS

Abstract

庄辉,TracyL,崔怡辉,凌斌华,张玲霞,BowdenS.我国部分地区丙型肝炎病毒基因分型研究[J].Chinese journal of Epidemiology,2001,22(2):99-101

我国部分地区丙型肝炎病毒基因分型研究

Study on hepatitis C virus genotyping in some parts of China

Received:July 19, 2000

DOI：

KeyWord: 丙型肝炎病毒丙型肝炎病毒核糖核酸基因型

English Key Word: Hepatitis C virus HCV RNA Genotype

FundProject:

Author Name	Affiliation
ZHUANG Hui	北京大学基础医学院病原生物学系 100083
TRACY L	澳大利亚维多利亚州立传染病参比实验室
CUI Yihui	北京大学基础医学院病原生物学系 100083
LING binhua	北京大学基础医学院病原生物学系 100083
ZHANG lingxia	解放军第三★二医院
Bowden S	澳大利亚维多利亚州立传染病参比实验室

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Abstract:

目的研究中国丙型肝炎病毒(HCV)基因型分布。方法应用AmplicorPCR试剂盒检测北京、青岛、固安和周口等地27例抗HCV阳性的单采浆供血员和36例慢性丙型肝炎病人血清HCVRNA，并对其中36例HCVRNA阳性者用INNO-LiPA^TMHCVⅡ试剂盒进行HCV基因分型。结果抗HCV阳性的单采浆供血员和慢性丙型肝炎病人HCVRNA检出率分别为48.15%(13/27)和69.44%(25/36)，平均为60.32%(38/63)。对13例HCVRNA阳性的单采浆供血员和23例慢性丙型肝炎病人HCV基因分型结果表明，29例(80.55%)为1b型，5例(13.89%)为2型，另2例(5.56%)不能被分型。结论上述调查地区流行的HCV基因型以1b型占优势。

English Abstract:

Objective To study the genotypes of hepatitis C virus in some parts of China.Methods Twenty-seven sera of plasma donors from Guan county of Hebei province, Zhoukou district of Henan province, and 36 patients with chronic hepatitis C from Beijing and Qingdao cities were tested for HCV RNA by Amplicor PCR kit.Thirty -six of 38 HCV RNA positive sera were further genotyped, using INNO -LiPA^TMHCV Ⅱ kit.Results The HCV RNA positive rates of plasma donors and patients with chronic hepatitis C were 48. 15%( 13/ 27)and 69. 44%( 25/ 36)respectively.Genotyping data from 13 plasma donors and 23 patients with chronic hepatitis C showed that 29( 80. 55%)were 1b, 5( 13. 89%)were genotype 2, and 2 ( 5. 56% )remained untyped.Conclusion 1b seemed to be the prevalent HCV genotype in some parts of China.

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