| 赖世隆,陈云波,温泽淮,侯孟君,梁伟雄,王奇,杨志敏.广州75岁以上老年人群中载脂蛋白E基因多态性与阿尔茨海默病关系的研究[J].Chinese journal of Epidemiology,2001,22(3):202-204 |
| 广州75岁以上老年人群中载脂蛋白E基因多态性与阿尔茨海默病关系的研究 |
| Association between apolipoprotein E polymorphismand Alzheimer' s disease:Apopulation-based study in Guangzhou, China |
| Received:October 15, 2000 |
| DOI： |
| KeyWord: 阿尔茨海默病 载脂蛋白E类 等位基因 基因频率 |
| English Key Word: Alzheimer's disease Apolipoprotein E Alleles Gene frequency |
| FundProject:广东省科委自然科学基金(930914)和广东省卫生系统“五个一科教兴医工程”重点科研项目资助(粤卫科[ 1996] 20) |
| Author Name | Affiliation | | LAI Shilong | Guangzhou University of Tradition Chinese Medicine, Guangzhou 510405, China | | CHEN Yunbo | Guangzhou University of Tradition Chinese Medicine, Guangzhou 510405, China | | WEN Zehuai | Guangzhou University of Tradition Chinese Medicine, Guangzhou 510405, China | | 侯孟君 | 广东省中医院老年脑病研究所 | | 梁伟雄 | Guangzhou University of Tradition Chinese Medicine, Guangzhou 510405, China | | 王奇 | Guangzhou University of Tradition Chinese Medicine, Guangzhou 510405, China | | 杨志敏 | 广东省中医院老年脑病研究所 |
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| Abstract: |
| 目的 了解广州地区高龄老年人群中阿尔茨海默病 (AD)患者载脂蛋白E(apoE)基因型的分布,探讨apoEε4等位基因和AD之间的关联性。方法 采用系统整群抽样方法获取样本进行社区横断面研究。调查广州市荔湾区 75岁以上的老年居民 382 5名,按DSM Ⅲ R和NINCDS/ADRDA的诊断标准,从中筛选出无血缘关系的散发性AD患者 190例,其中 110例接受抽血检查,并以该人群中 5 18名接受抽血检查的健康老年人作为对照组。用PCR RFLP技术,检测以上两组的apoE基因型。 结果 AD组apoEε2、ε3、ε4等位基因频率分别为10.0 %、83.2 %、6.8%,对照组apoEε2、ε3、ε4等位基因频率分别为 9.2 %、84.3%、6.5 %。AD组与对照组在apoE基因型频率及等位基因频率分布上的差异均无显著性 (P >,0.0 5 )。结论 广州市荔湾区 75岁以上老年人群中,未发现apoEε4等位基因与散发性AD之间有显著性关联 |
| English Abstract: |
| Objective To investigate the distribution of apolipoprotein E (apoE)genotype of patients with Alzheimer' s disease(AD)in the population aged 75 andolder in Guangzhou and to determine whether there was an association between AD and apoEε4 allele.MethodsA systematic cluster sampling design, two-stage screening and field evaluations were used in a cross-sectional population-based study. Three thousand eight handred and twenty-five subjects aged 75 and above in Liwan district of Guangzhou were interviewed.Blood samples were collected from 110 cases and 518 normal controls.The criteria of DSM-Ⅲ-R and NINCDS-ADRDA were used for clinical diagnosis of probable AD.Polymerase chain reaction(PCR)and restriction fragment length polymorphism (RFLP)were used to determine apoE genotypes.ResultsThere were 190 cases diagnosed as sporadic AD from this sample.The frequencies of the three apoE alleles were detected as follows:ε2=0.100, ε3=0.832, ε4=0.068 in AD patients;ε2=0.092, ε3=0.843, ε4=0.065 in normal controls.The frequencies of apoE genotype and allele in the AD patients were not significantly different from that in normal subjects (P >0.05).Conclusion No association between sporadic AD and apoE ε4 allele was found among the population aged 75 and above in Liwan district of Guangzhou. |
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