| 董兴齐,叶枫,彭何碧,李俊勇,高子厚,尹家祥,张洪英,吴明寿.云南省鼠疫菌质粒特征及地理分布[J].Chinese journal of Epidemiology,2001,22(5):344-347 |
| 云南省鼠疫菌质粒特征及地理分布 |
| Geographic distribution and feature of Yersinia pestis plasmid isolated from Yunnan province |
| Received:December 23, 2000 |
| DOI： |
| KeyWord: 鼠疫菌 质粒 流行病学,分子 |
| English Key Word: Yersinia pestis Plasmid Epidemiology, molecular |
| FundProject:国家自然科学基金资助项目(39960006);云南省中青年学术和技术带头人培养经费资助项目(1999C0026G) |
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| Abstract: |
| 目的探讨云南省鼠疫菌质粒分子流行病学意义。方法按Kado和Liu法提取云南省44个县市及中缅边境一带共1020株鼠疫菌的质粒,经琼脂糖凝胶电泳进行检测分析。结果云南省鼠疫菌具有相对分子质量(Mr)分别约3.93、6.05、22.97、35.65、45.35、64.82、74.59、111.36和129.55×106的9种质粒,其中3.93、35.65和111.36×106的3种质粒为云南省鼠疫菌特有。按质粒组成可分为10种不同类型的质粒谱,其中Ⅰ～Ⅴ种类型较为常见,且在分布上具有明显的地区集中现象。结论根据鼠疫菌质粒特征,可将云南省自1982年以来发生鼠疫流行的地区划分为多片相对独立的疫源地。认为80年代重新开始的家鼠鼠疫流行,可能来源于多片潜伏的鼠疫自然疫源地的复燃。 |
| English Abstract: |
| Objective To analysis the molecular epidemiological feature of Yersinia pestis plasmid isolated from Yunnan. Methods One thousand and twenty strains of Y. pestis isolates from 44 counties of Yunnan province in China and the border of China Myanmar using Kado and Liu method and detected by agrose gel electrophoresis. Results Results showed that these strains from Yunnan province carried 9 kinds of plasmids with molecular weights about 3.93, 6.05, 22.97, 35.65, 45.35, 64.82, 74.59, 111.36 and 129.55 ×10 6. Of these, three kinds of 3.93, 35.65 and 111.36 ×10 6 were found very specific, only reported in Yunnan. The plasmid profile could be divided into 10 types according to the plasmid content. Type Ⅰ Ⅴ were common and had a clustering nature in geographic distribution. Conclusion Based on the plasmid profile, the epidemic districts of plague could be divided into several independent plague foci, suggesting that the resurgence of domestic rat caused plague epidemics might have several hidden plague foci as the result of epidemics since 1982 in Yunnan. |
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