乙型肝炎病毒基因型和血清亚型在我国部分地区的分布及其特点

夏国良; OmanaVNainan; 贾志远; 刘洪斌; 罗述斌; 李荣成; 曹慧霖; 刘崇柏; HaroldSMargolis

Abstract

夏国良,OmanaVNainan,贾志远,刘洪斌,罗述斌,李荣成,曹慧霖,刘崇柏,HaroldSMargolis.乙型肝炎病毒基因型和血清亚型在我国部分地区的分布及其特点[J].Chinese journal of Epidemiology,2001,22(5):348-351

乙型肝炎病毒基因型和血清亚型在我国部分地区的分布及其特点

Characterization and distribution of hepatitis B virus genotypes and subtypes in 4 provinces of China

Received:April 15, 2001

DOI：

KeyWord: 肝炎病毒,乙型流行病学,分子基因型血清亚型

English Key Word: Hepatitis Bvirus Molecular epidemiology Genotype Subtype

FundProject:国家“九五”科技攻关项目(969060302)

Author Name	Affiliation
XIA Guoliang	中国预防医学科学院病毒学研究所肝炎室北京,100052
Omana V Nainan	Hepatitis Branch
JIA Zhiyuan	河北省卫生防疫站
刘洪斌	湖南省卫生防疫站
罗述斌	广西壮族自治区卫生防疫站
李荣成	中国预防医学科学院病毒学研究所肝炎室北京,100052
曹慧霖	中国预防医学科学院病毒学研究所肝炎室北京,100052
刘崇柏	中国预防医学科学院病毒学研究所肝炎室北京,100052
HaroldSMargolis	中国预防医学科学院病毒学研究所肝炎室北京,100052

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Abstract:

目的研究乙型肝炎病毒(hepatitisBvirus,HBV)基因型和血清亚型的分布特点及其基因的相关性。方法湖南、广西、河南和河北4省(区)25个县(市)280例慢性HBV携带者,应用聚合酶链反应(PCR)扩增和脱氧核糖核酸(DNA)序列分析,确定HBV基因型和血清亚型。结果HBV基因型B、C和D均有分布,分别占29.3%、67.9%和2.9%,其中B和C为优势基因型。adr、adw2、ayr、ayw1、ayw2和ayw3等6种血清亚型均有分布,其中adr和adw2为优势血清亚型,分别占64.3%和31.4%。基因型B与adw2血清亚型,基因型C与adr血清亚型有非常密切的基因相关性。280例HBV携带者表面抗原基因序列每100个核苷酸的平均置换频数为2.94。基因型B(adw2血清亚型)毒株核苷酸置换频数为5.63(5.48),而基因型C(adr血清亚型)仅为1.6(1.51)。结论HBV基因型和血清亚型的分布均有明显的地区性;基因型B及相对应的adw2血清亚型毒株的基因可变性明显高于基因型C及相应的adr血清亚型。

English Abstract:

Objective To determine the distribution and genetic relationship of hepatitis B virus (HBV) genotypes and subtypes. Methods HBV genotypes and subtypes were determined by PCR and DNA sequencing among 280 chronic HBV carriers in 25 counties of 4 provinces (Hunan, Guangxi, Henan and Hebei) in China. Results Genotype B, C and D were detected in these regions. Genotypes C and B were the majority genotypes of HBV with 190 out of 280 ( 67.9 %) genotype C, 82 ( 29.3 %) genotype B, and 8 ( 2.9 %) genotype D. Adr, adw2, ayr, ayw1, ayw2 and ayw3 subtypes were determined among these carriers. Adr and adw2 subtypes were the leading subtypes of HBV,taking up 64.3 % and 31.4 %, respectively.Adr subtype was completely encoded by genotype C while majority of adw2 subtype was encoded by genotype B.An average rate nucleotide substitutions of 2.94 was seen among 280 Chinese HBV sequences. The average rate of nucleotide substitutions of genotype B (adw2 subtype) was 5.63 ( 5.48 ), but only 1.6 ( 1.51 ) for genotype C (adr subtype). Conclusion The results suggested that there were significant differences in geographic distribution of HBV genotypes and subtypes; genotype B, in which mostly consistent with adw2 subtype, was a higher variable than genotype C (adr subtype).

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