| 韩晓旭,姜拥军,尚红,王亚男,卢春明,张世博,姜凤霞,邵一鸣.辽宁省流行的人类免疫缺陷病毒1基因亚型调查[J].Chinese journal of Epidemiology,2001,22(6):432-434 |
| 辽宁省流行的人类免疫缺陷病毒1基因亚型调查 |
| Genetic subtyping of HIV-1 in Liaoning province of China |
| Received:June 30, 2001 |
| DOI： |
| KeyWord: 人类免疫缺陷病毒-1 亚型 前病毒脱氧核糖核酸 异源双链泳动分析 |
| English Key Word: Subtype of human immunodeficiency virus-1 Proviral deoxy ribo nucleic acid Heteroduplex analysi |
| FundProject:广东省卫生厅科研基金资助项目 (A2000316) |
| Author Name | Affiliation | | HAN Xiaoxu | P3 Laboratory, The 1st Affiliated Hospital China Medical University, Shenyang 110001, China | | JIANG Yongjun | P3 Laboratory, The 1st Affiliated Hospital China Medical University, Shenyang 110001, China | | SHANG Hong | P3 Laboratory, The 1st Affiliated Hospital China Medical University, Shenyang 110001, China | | 王亚男 | P3 Laboratory, The 1st Affiliated Hospital China Medical University, Shenyang 110001, China | | 卢春明 | 辽宁省疾病预防与控制中心性病控制科 | | 张世博 | 辽宁省疾病预防与控制中心性病控制科 | | 姜凤霞 | 辽宁省疾病预防与控制中心性病控制科 | | 邵一鸣 | 卫生部艾滋病预防与控制中心参比实验室 |
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| Abstract: |
| 目的了解辽宁省人类免疫缺陷病毒(human immuno deficiency virus-1，HIV-1)各亚型的流行情况。方法 采集1997 年6 月至2000 年12 月间辽宁省检出的16 例HIV-1 感染者的全血标本，提取核酸后用巢式-聚合酶链反应(nested-PCR)技术扩增HIV-1 env 基因区， 用异源双链泳动分析法(he teroduplex mobility assay, HMA)进行HIV-1 亚型的分析。结果 辽宁省目前已有HIV-1A 、B' 、C 和E 亚型流行。经性途径感染占全部HIV 感染的31.25 %， 分别为泰国B(B' )亚型和A 亚型，以B'为主;经注射吸毒感染占全部HIV 感染31.25 %，分别为C 亚型和E 亚型， 以C 亚型为主， 其中1 名吸
毒者为B 或E 亚型：经输供血途径感染占全部HIV 感染的31.25 %，主要为泰国B' 亚型， 也可见C 亚型：垂直传播1 名为A 亚型。结论 辽宁省已有多种亚型HIV-1 毒株流行， 有效地控制艾滋病经性乱、吸毒和输供血途径传播的问题已迫在眉睫。 |
| English Abstract: |
| Objective To study the prevalence of HIV-1 in Liaoning province of China.Methods Nuclear acids were extracted from blood samples of 16 HIV-1 infected individuals collected locally in Liaoning province of China from Jun.1997 to Dec.2000.The 0.7 kb or 1.2 kb segments of HIV-1 envgene were amplified using nested-PCR and the HIV-1 genetic subtypes were then assayed by heteroduplex mobility assay.Results Fifteen of 16 samples were positive by PCR amplification of HIV-1 env region and samples were found to be genetic subtype A, B', C, E.The propor tion due to sexual transmissio n in all HIV infection was 31.25 % (5/15), among w hich subty pe B' (3/5)w as the majority.A man who returned f rom Africa together with his spouse both had type A(2/ 5)infection.I ntravenous drug users (IDUs)took up 31.25 %(5/15)of all the HIV infections.Subtype C (2/4)and E were predominant among intravenous drug users.However, there w as one IDU with subtype B or E.Nearly all blood recipients and blood donors
w ere B' (4/5)ex cept one with C.Conclusion There have been several subty pes of HIV-1 existed in
Liaoning province, demonstrating the complexity of HIV epidemolo gy in Liaoning pro vince and the
difficulty conducting prevention and treatment |
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