| 彭义利,汤兵祥,刘俊刚,甄新安,王翠花,王国斌.河南省0 ～ 14 岁儿童支气管哮喘流行病学调查[J].Chinese journal of Epidemiology,2001,22(6):470-471 |
| 河南省0 ～ 14 岁儿童支气管哮喘流行病学调查 |
| NIES-90 microcystin producing algae strain genome library construction and one isolated gene analysis |
| Received:July 16, 2001 |
| DOI： |
| KeyWord: 微囊藻毒素 基因组文库 |
| English Key Word: Microcystin Genome plasmid library |
| FundProject:河南省科技攻关计划项目(001170412) |
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| Abstract: |
| 为了解河南省支气管哮喘(哮喘)的发病情况及相关因素, 我们于2000 年3 ～ 6 月进行了全省全人群哮喘流行病学调查(随全省结核病流行病学调查同步进行)。现将其中0 ～
14 岁儿童哮喘流行病学调查(流调)结果报告如下。 |
| English Abstract: |
| ObjectiveTo construct a plasmid microcystis genome library for microcystin (MC) pertaining gene family screening or microcystis strain specific gene screening. Methods Extracting genome DNA from MC producing strain NIES 90, then digesting DNA by HindⅢ and ligating to CIPase treated pUC18/ HindⅢ fragment which later transformed to JM109. After checking the library by Xgal/Antibiotics plate, clones isolation, a few clones were sequnenced and specific primers synthesized; finally, PCR was used to testify clone's NIES 90 strain specificity. Results Successfully constructed a NIES 90 genome plasmid library with following functions: a) cloning efficiency more than 95% by comparing the white/blue clones ratio in Xgal Plate; b) 200 700 bp average insert length with 3×10 5 independent clones by independent clones analysis; c) 10 times more coverage of original NIES 90 genome; d) strong strain specificity by cross PCR analysis of some clone's sequnence in two different microcystis strains compared with FACHB 469. Conclusion We successfully constructed a NIES 90 MC producing microcystis strain's genome plasmid library which could be used for screening MC pertaining gene family because of high strain specificity of some clones. |
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