| 韩秀兰,林惠芳,孟宗达,杜敏,侯通,杨翠丽,董永辉,赵玉良,刘洪斌,曹惠霖,刘崇柏.乙型肝炎病毒基因重组疫苗阻断母婴传播的进一步观察[J].Chinese journal of Epidemiology,2002,23(2):118 |
| 乙型肝炎病毒基因重组疫苗阻断母婴传播的进一步观察 |
| Molecular epidemiology on vpr gene of HIV 1 strains |
| Received:July 26, 2001 Revised:August 15, 2012 |
| DOI： |
| KeyWord: 人类免疫缺陷病毒1型 vpr基因 基因多态性 |
| English Key Word: Human immunodeficiency virus type 1 vpr gene Gene polymorphism |
| FundProject:国家"九五"科技攻关项目(96-90603) |
| Author Name | Affiliation | E-mail | | 韩秀兰 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | wxhszen@gmail．Eom | | 林惠芳 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | | | 孟宗达 | Shenzhen Futian District Center for Disease Control and Prevention | | | 杜敏 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | | | 侯通 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | | | 杨翠丽 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | | | 董永辉 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | | | 赵玉良 | Shenzhen Futian District Center for Disease Control and Prevention | | | 刘洪斌 | Shenzhen Futian District Center for Disease Control and Prevention | | | 曹惠霖 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | | | 刘崇柏 | Shenzhen Municipal Center for Disease Control and Prevention,Shenzhen 518020,China | |
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| Abstract: |
| "七五"和"八五"计划期间,我国对第一代乙型肝炎(乙肝)疫苗(血源疫苗)的新生儿免疫效果进行了近期和远期效果考核,均证明效果良好."九五"期间又对第二代乙肝疫苗(基因工程疫苗)效果进行考核,以最后取代血源疫苗.本文报道对我国研制的在哺乳动物细胞中高效表达的基因工程疫苗(CHO疫苗)阻断母婴传播的初步结果. |
| English Abstract: |
| Objective To understand the rule of vpr gene variance of HIV 1 strains. Methods RT PCR was used to amplify vpr gene of HIV 1 strains in Shenzhen. PCR products were sequenced and used for gene phylogenetic analysis and the 32 46 amino acids of Vpr protein were compared. The difference of 77 amino acid polymorphism distribution between domestic region and foreign region was analyzed. Results 01_AE was the major HIV 1 subtype in Shenzhen. The gene distance among subtype B was larger than in other subtypes. 77 amino acid of Vpr protein had three polymorphism forms as Arginin, Glutamine and Histidine, with Glutamine as the wild form. There were no significant differences in the three amino acid distributions between HIV 1 strains from domestic region and foreign region. Conclusion vpr genes of different HIV 1 strains belonged to 01_AE subtype. There was polymorphism seen in the vpr gene which was consistent with both domestic and international HIV 1 strains. |
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