| 唐青,高佃平,赵秀芹,韩磊,杭长寿.5株新疆出血热病毒分子流行病学研究[J].Chinese journal of Epidemiology,2002,23(6):449-452 |
| 5株新疆出血热病毒分子流行病学研究 |
| Study on the molecular biology of hemorrhagic fever virus in Xinjiang |
| Received:November 30, 2001 |
| DOI： |
| KeyWord: 新疆出血热|S基因|同源性|流行病学,分子 |
| English Key Word: Xinjiang hemor rhagic fever|S gene|Homology|Epidemiology, molecular |
| FundProject:国家自然科学基金资助项目 (39870680 ) |
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| Abstract: |
| 目的 从分子水平揭示中国新疆出血热 (XHF)病毒基因结构与功能的关系,寻找传播途径及流行原因。方法 对分离自新疆的 5株XHF病毒进行S基因片段的克隆和测序,与其他克里米亚 刚果出血热 (CCHF)的S基因序列进行比较和分析。 结果 5株病毒S基因全长均为 1672个核苷酸, 开放阅读框均为 14 4 9个核苷酸,编码一个含 482个氨基酸的蛋白。 新疆分离毒株的S基因核苷酸序列同源性 (93.0%～ 99.5% )明显高于其他国家分离毒株的S基因核苷酸序列。 系统发生树状图显示新疆毒株在一个分支之下形成独立的群体,明显区别于来自其他地区的CCHF病毒并且又进一步分为三组。结论 不同毒株的S基因序列差异不完全取决于病毒分离的宿主、地域和时间。 |
| English Abstract: |
| Objective To explore the relationship between the structure and function at molecular level and the routes of transmission of Xinjiang hemorrhagic fever(XHF)virus.Methods S genes of five XHF virus strains were cloned, sequenced and compared with that of other Crimean-Congo hemorrhagic fever virus strains.Results It was found that S genes of the five viruses had 1672 nuclei tides, while ORF of them including 1449 nuclei tides and coded with a protein of 482 amino acid.The nucleotides homology of Chinese isolates (93.0 %-99.5 %)was obviously higher than that of any other S genes strains identified in other countries'.Phylogenetic tree showed that all Chinese isolates clustered into one branch and could be further divided into another three groups.Conclusion The sequencial difference of S genes was not to tally related to the host, areas and time of the viruses isolated. |
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