| 丁晓萍,闫素文,张宁,董卫,鲁海鸥,汤洁,王喜良.寒冷地区部队指战员精液质量的调查[J].Chinese journal of Epidemiology,2003,24(3):206-209 |
| 寒冷地区部队指战员精液质量的调查 |
| Study on sperm quality of males living in cold area |
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| DOI： |
| KeyWord: 精液质量 寒冷地区 单细胞凝胶电泳 染色质 |
| English Key Word: Semen quality Cold area Single-cell gel electrophoresis Chromatin assay |
| FundProject:全军“十五”医药卫生科研基金重点课题资助项目(01Z002) |
| Author Name | Affiliation | | DING Xiaoping | Reproduction Center, 202nd Hospital of Shenyang Command, People′s Liberation Army, Shenyang 110003, China | | YAN Suwen | Reproduction Center, 202nd Hospital of Shenyang Command, People′s Liberation Army, Shenyang 110003, China | | ZHANG Ning | Reproduction Center, 202nd Hospital of Shenyang Command, People′s Liberation Army, Shenyang 110003, China | | DONG Wei | 中国医科大学临床流行病学教研室 | | LU Haiou | Reproduction Center, 202nd Hospital of Shenyang Command, People′s Liberation Army, Shenyang 110003, China | | TANG Jie | Reproduction Center, 202nd Hospital of Shenyang Command, People′s Liberation Army, Shenyang 110003, China | | WANG Xiliang | Reproduction Center, 202nd Hospital of Shenyang Command, People′s Liberation Army, Shenyang 110003, China |
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| Abstract: |
| 目的探讨在寒冷地区作业是否对精液质量产生影响。方法通过现况调查,采用分子水平实验技术即单细胞凝胶电泳和染色质结构分析与精液常规检查相结合的方法,评估寒冷地区部队指战员精液质量和精子亚临床损伤。结果寒冷地区作业人员精液常规化验均在正常范围,但单细胞凝胶电泳Ⅰ级彗星率高达4.4%,与对照组(1.9%)比较差异有显著性;精子染色质结构分析中compαt增高,平均值为22.26%,两种实验结果均反映了精子DNA单双链不同程度的损伤。结论长期工作在寒冷地区的人员可见精子DNA单双链损伤增加,但不足以影响精液质量。提示加强寒冷地区男性作业人员的生殖健康保健十分必要 |
| English Abstract: |
| Objective To investigate the effects on male reproductive function working under cold area. Methods After on site investigation, advanced molecular lab analysis-single-cell gel electrophoresis (SCGE) and sperm chromatin structure assay (SCSA) which are combined with semen routine analysis were used to evaluate semen quality and sperm sub-clinical injury. Results Semen routine analysis showed that the semen parameters of the males working in cold area were within normal range, but level Ⅰ comet cell percentage in SCGE increased significantly, which was 4.4%, compared to the contrast group ( 1.9%) with significant difference. During sperm chromatin structure assay parameters, compat increased, with an average value of 22.26%. The two kinds of results both showed single and double strand breakages in sperm. Conclusion Long-term exposure to cold could induce sperm DNA injury, but not affect sperm quality. The results suggested that it was important to reinforce the reproductive care in males working in cold areas. |
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