北京、广东、宁夏三地结核分支杆菌DNA指纹的应用研究

李卫民; 王苏民; 裴秀英; 刘忠泉; 钟球; 钱明; 赵冰; 端木宏谨

Abstract

李卫民,王苏民,裴秀英,刘忠泉,钟球,钱明,赵冰,端木宏谨.北京、广东、宁夏三地结核分支杆菌DNA指纹的应用研究[J].Chinese journal of Epidemiology,2003,24(5):381-384

北京、广东、宁夏三地结核分支杆菌DNA指纹的应用研究

DNA fingerprinting of Mycobacterium tuberculosis strains from Beijing,Guangdong and Ningxia

Received:September 10, 2002

DOI：

KeyWord: 结核杆菌 DNA指纹法聚类分析

English Key Word: Mycobacterium tuberculosis DNA fingerprints Cluster analysis

FundProject:

Author Name	Affiliation
LI Wei-min	Beijing Tuberculosis and Chest Tumor Institution,Beijing 101149,China
WANG Su-min	Beijing Tuberculosis and Chest Tumor Institution,Beijing 101149,China
PEI Xiu-ying	宁夏回族自治区医学院
LIU Zhong-quan	Beijing Tuberculosis and Chest Tumor Institution,Beijing 101149, China
ZHONG Qiu	广东省结核病防治所
QIAN Ming	广东省结核病防治所
ZHAO Bing	Beijing Tuberculosis and Chest Tumor Institution,Beijing 101149,China
DUANMU Hong-jin	Beijing Tuberculosis and Chest Tumor Institution,Beijing 101149,China

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Abstract:

目的分析“北京家族”结核分支杆菌,从分子流行病学角度探讨北京、广东及宁夏三地结核分支杆菌的分布特征。方法采用Gelcompare4.1软件对来自三地的206株结核分支杆菌的插入序列IS6110DNA指纹图谱进行数字化,经互联网与世界结核分支杆菌DNA指纹库进行相似性比较;同时应用该软件对上述菌株行聚类分析;构建标准的结核分支杆菌Spoligotyping(间隔区寡核苷酸分型法)DNA指纹方法;用χ²检验比较不同组别肺结核病人临床分离菌株成簇率的差别,同时计算相对危险度(OR)。结果206株结核分支杆菌的IS6110DNA指纹图谱与DNA指纹图谱库相比较,未发现相同者。56.8%(117/206)的结核分支杆菌IS6110DNA指纹相似值在1.00～0.65之间,且它们的Spoligotyping指纹图谱均与“北京家族”结核分支杆菌相一致;分组统计显示:男性组与女性组、年龄≥42岁组与＜42岁组成簇率之间差异有显著性(P＜0.05),OR值为男性4.43,95%CI:0.94～28.76;＜42岁为5.06,95%CI:1.00～34.34。其他各组之间差异无显著统计学意义(P＞0.05)。结论“北京家族”结核分支杆菌在三地呈较高水平的流行;结核分支杆菌在男性和＜42岁人群中的传播频率较女性和≥42岁人群为高,且男性和＜42岁可能是三地结核病近期传播的危险因素。利用结核分支杆菌的DNA指纹图谱和互联网技术,可以实现结核分支杆菌传染源全球范围的追踪。

English Abstract:

Objective　To explore the epidemic distribution of Mycobacterium tuberculosis isolates from Beijing,Guangdong and Ningxia,and to determine M.tuberculosis strains of the“ Beijing Family”.Methods　Two hundred and six IS6110 DNA fingerprinting patterns of M.tuberculosis strains from three provinces (city) were transferred to digital data,ompared with the world M.tuberculosis DNA fingerprinting database,and then clustered by Gel compare 4.1 software.The clustering valuesin different patients with tuberculosis were compared by χ² test.Risk factors for recent transmission were calculated using odd ratios.Results No M.tuberculosis strains were found the same as those of DNA fingerprint database.56.8%(117/206)fingerprinting patterns of M.tuberculosis shared by least two-thirds of the IS6110 fragments and their Spoligotyping fingerprinting patterns were consistent with those of M.tuberculosis strains of the“ Beijing Family” .There were significant differences between female and male,different age groups (<42 years old)and older (≥42 years old)(P<0.05).Odd ratio was 5.06 in the group younger than 42 years old (95%CI:1.00-34.34)and w as 4.43 (95%CI:0.94-28.76)in males. Conclusion　M.tuberculosis strains of “Beijing Family” were popular in Beijing, Guangdong and Ningxia.Men and younger age group (<42)were shown to be infected by identical strains more often than women and older aged which might play an important role in the recent transmission of tuberculosis in these areas.IS6110 DNA fingerprinting of M.tuberculosis could be used to trace the source of tuberculosis infection .

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