| 何敏,曾尔良,郑艳燕,汤卓,卢祥婵,孙碧辉,许丁空,张志勇,杨莉.利用基因芯片检测结核分支杆菌及其利福平耐药性的研究[J].Chinese journal of Epidemiology,2003,24(5):385-388 |
| 利用基因芯片检测结核分支杆菌及其利福平耐药性的研究 |
| Identification of Mycobacterium tuberculosis and rifampin-resistant strains by gene-chips |
| Received:October 20, 2002 |
| DOI： |
| KeyWord: 结核杆菌 耐药 基因芯片 |
| English Key Word: Mycobacterium tuberculosis Drug-resistance Gene-chip |
| FundProject:广西科技厅资助项目 (桂科攻023502427) |
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| Abstract: |
| 目的探讨结核杆菌耐利福平(RFP)检测基因芯片在结核病诊断及其耐药性检测中的应用价值。方法采用结核杆菌耐RFP芯片对35株RFP耐药的临床分离菌株,102例肺结核患者、27例非结核病的其他患者的痰标本中的结核杆菌及其RFP耐药性进行了检测,基因芯片检测结果与痰涂片、细菌培养及结核杆菌标准化药敏试验结果比较。结果结核杆菌耐RFP检测芯片检测35株耐药株,有33株判定为RFP耐药株,与传统药敏试验结果的符合率为94.29%。对27份其他患者的痰液标本进行结核杆菌检测,特异性为92.59%。对102份结核患者痰标本中结核杆菌进行检测,痰涂片法阳性率35.29%(36/102),细菌培养法阳性率28.43%(29/102),基因芯片法阳性率77.45%(79/102)。传统的药敏试验报告102份痰标本仅29份培养出结核分支杆菌,其中8份RFP耐药株,而基因芯片法检测102份痰标本中发现20份耐RFP结核杆菌。主要基因突变位点为531、526和516。结论采用结核杆菌耐RFP检测芯片检测结核杆菌及RFP耐药性具有快速简便、特异性高的特点。 |
| English Abstract: |
| Objective To evaluate the gene-chip detecting rifaman-resistance Mycobacterium tuberculosis applied in TB diagnosis and drug resistant dectection.Methods Mycobacterium tuberculosis and rifaman-resistant strains among 35 rifaman-resistance isolated strains and 102 sputa specimens from TB patients,27 sputa specimens from other patients were examined the gene-chips.Results obtained werecompared with sputum examination,bacteriological culture and standard drug susceptibility test of Mycobacterium tuberculosis.Results Thirty-five rifaman-resistance strains were detected by gene-chips and 33 were identified as rifaman-resistance strains and the concordance with the traditional drug susceptibility test of Mycobacterium tuberculosis was 94.29%.Twenty-seven sputa specimens from other patients were examined Mycobacterium tuberculosis by the gene-chips,2 were positive,the detection specialty was 92.59%.Using three methods detecting Mycobacterium tuberculosis among 102 sputa specimens the positive rate respectively was,sputum examination 35.29% (36/ 102),bacteriologicalculture 28.43%(29/102),gene-chip 77.45%(79/ 102).Among 102 sputa specimens only 29 examined Mycobacterium tuberculosis by the traditional drug susceptibility test and 8 were rifaman-resistant strains.While using gene-chip,there were 20 among 102 sputa specimens identified as rifaman-resistance strains.Among total 55 rifaman-resistance strains detected by the gene-chips,the most frequent mutations were those associated with codon 531 (23 of 55;41.8%),526(15 of 55;27.27%)and 516(9 of 55;16.36%).Conclusion Results showed that this was a rapid,simple and highly specific method when using gene-chip to detect Mycobacterium tuberculosis and rifaman-resistant strains. |
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