| 杨韬,文静波,沈庆辉,李云涛,翟珏明,李茂云.昆明市某山村小学水痘爆发的调查[J].Chinese journal of Epidemiology,2003,24(6):457 |
| 昆明市某山村小学水痘爆发的调查 |
| Surveillance of Escherichia coli O157：H7 among animals in Jiangsu province in 1999 |
| Received:January 28, 2003 Revised:June 18, 2012 |
| DOI： |
| KeyWord: 大肠杆菌O157：H7 宿主动物 带菌率 毒力基因 |
| English Key Word: Escherichia coli O157：H7 Host animal Carrier rate Toxic gene |
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| Abstract: |
| 2002年5～7月昆明市嵩明县某山村小学爆发一起水痘. |
| English Abstract: |
| Objective To understand the Escherichia coli O157：H7 carrier rate of host animals and the toxic gene of the strains in different areas in Jiangsu province. Methods Surveillance spots were set up in different areas, to collect feces of pigs, chickens, sheep, cattle to culture for O157：H7 with immunomagnetic separation as well as detection of toxic gene of the strain with MPCR were both carried out. Results One hundred and seventy strains of O157：H7 were separated from 1 767 feces of different animals in six spots,with a overall positive rate 9.62 %. The positive rates of cattle and sheep were 19.05 % and 12.01% respectively. Among 85 strains SLT1,SLT2, eaeA and hly toxic genes were detected. In which, 56.47 % of the strains were positive curturely while 79.17 % of them carried SLT2, eaeA and hly gene simultaneously. Conclusion The positive rate of O157：H7 in animals and the positive rates of strains were correlated to the incidence of the area. The highest rates were seen in areas where there had been O157：H7 epidemic, followed by the areas where there were only scattered cases identified while the lowest was in areas with no patients. Data indicated that it was important to enforce the surveillance of O157：H7 in animals to better predict and control of the disease. |
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