胸苷酸合成酶基因多态和生活习惯及亚甲基四氢叶酸还原酶基因协同作用与胃癌的易感性

高长明; 吴建中; 刘燕婷; 丁建华; 李苏平; 苏平; 胡旭; 开海涛; Takezaki Toshiro; Tajima Kazuo

Abstract

高长明,吴建中,刘燕婷,丁建华,李苏平,苏平,胡旭,开海涛,Takezaki Toshiro,Tajima Kazuo.胸苷酸合成酶基因多态和生活习惯及亚甲基四氢叶酸还原酶基因协同作用与胃癌的易感性[J].Chinese journal of Epidemiology,2003,24(7):599-603

胸苷酸合成酶基因多态和生活习惯及亚甲基四氢叶酸还原酶基因协同作用与胃癌的易感性

Interactions between lifestyle thymidylate synthase gene with methylanetetrah川rofolate reductase generisk of stomach cancer

Received:October 10, 2002

DOI：

KeyWord: 胃肿瘤胸苷酸合成酶基因多态性吸烟饮酒饮茶

English Key Word: Stomach neoplasms Thymidylate synthase genotypes Smoking Alcohol drinking Tea drinking

FundProject:日本文部省国际学术研究癌症特别研究经费资助项目(08042015)

Author Name	Affiliation
Gao Changming	Department of Epidemiology Jiangsu Province
Wu Jianzhong	Department of Epidemiology Jiangsu Province
Liu Yanting	Department of Epidemiology Jiangsu Province
Ding Jianhua	Department of Epidemiology Jiangsu Province
Li Suping	Department of Epidemiology Jiangsu Province
Su Ping	Department of Epidemiology Jiangsu Province
Hu Xu	Institute of Cancer Research, Nanjing 210009, China
Kai Haitao	Institute of Cancer Research, Nanjing 210009, China
Takezaki Toshiro	日本爱知县がんセンタ一研究所疫学预防部
Tajima Kazuo	日本爱知县がんセンタ一研究所疫学预防部

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Abstract:

目的　研究胸苷酸合成酶(TS)3'-UTR基因多态和生活习惯及亚甲基还原酶基因相互作用与胃癌易感性的关系.方法　在上消化道癌高发区淮安市进行病例对照研究(胃癌患者107例,人群对照200名),调查研究对象的生活习惯,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测研究对象的TS 3'-UTR基因型.结果(1)胃癌组中TS +6bp/ +6bp、+6bp /-6bp、-6bp/ -6bp基因型频度分别为5.6 %、47.7%和46.7%,与对照组的9.0 %、54 .0 %和 37.0 %相比,差异无显著性(χ²=3.190,P=0.20 3).与携带 +6bp等位基因者相比,携带TS-6bp/-6bp基因型者胃癌发生的危险性有所升高(调整OR=1.36,95 %CI:1.00～1.78,P =0.047).(2)TS -6bp -6bp基因型对吸烟、饮酒及不饮茶的习惯在胃癌发生中的作用有放大效应.与携带 +6bp等位基因且不吸烟者或不经常饮酒者或饮茶者相比,携带-6bp/-6bp基因型且有吸烟习惯者或经常饮酒者或不饮茶者发生胃癌的调整OR分别为2.79 (95 %CI :1.51～5.18)、1.76(95 %CI:1.07～2.90)和2.34(95 %CI :1.43～ 3.82).(3)TS-6bp/-6bp基因型与亚甲基四氢叶酸还原酶(MTHFR)C/T或T/T基因型有协同作用.携带TS3'-6bp/ -6bp基因型同时携带MTHFR T等位基因者发生胃癌的调整OR为2.67(95 %CI:1.07 -6.70)结论TS 3'-UTR一6 bp/一6 bp基因型与生活习惯、MTHFR C/T或T/T基因型与胃癌易感性有明显的协同作用.

English Abstract:

Objective To evaluate interactions between lifestyle methylanetetrahydrofolate reductase gene(MTHFR) and polymorphisms in the 3’一untranslated region(3'-UTR) of the thymidylate synthase gene(TS) with reference to development of stomach cancer(SC).Method-s We conducted a casecontrol study with 107 cases of SC and 200 population-based controls in Huaian city of Jiangsu province China. TS genotypes were identified by polymerase chain reaction.Results(1)The frequencies of TS genotypes(+6 bp/+6 bp}+6 by/一6 by and一6 by/一6 by ) among the cases were 5.6%,47.7% and 46.7% and among the controls were9.0%,54.0% and 37.0%,resp-ectively.Individuals identified as一6 by/一6 by genotype had a slightly higher risk for SC than those individuals wit +6 bpalleles (the crude OR=1 .49) 95%CI:0.90-2.47; adjusted OR=1.36} 95%CI:1.00- 1.78(P=0.047).(2)Individuals hav-ing TS一h by/一6 by genotype and having smoking habit were at a significantly higher risk of developing SC(adjusted OR=2. 79 95%CI:1.51-5.18 )compared with those who had+6 by alleles with no smoking habit.Individuals having TS一6 bp/一6 bp genotype and habit of frequent alcohol drin-king were at an increased risk of developing SC(adjusted OR=1.76 95%CI:1.07-2.90) compared with those with+6 by alleles and low consumption of alcohol.As compared with individuals with +6 bp alleles and who had habit of tea drinking individuals who had TS一6 bp/一6 bp genotype and but without habit of tea drinking had an increased risk of developing SC(ad-justed OR=2.34 95%CI:1.43-3.82).C3)Individuals with TS一6 by/一6 by genotype and with MTHFR T alleles had an increased risk of developing SC(adjusted OR=2.67 95%CI:1.07-6.70)compared with those with+6 by alleles and with MTHRF genotype. Conclusion Results in the present study suggested that there was acombined effect between lifestyle MTHFR C/T or T/T genotype and TS一6 bp/一6 bp genotype in thedevelopment of SC.

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