| 陈建,万康林.中国莱姆病螺旋体PD91重组OspC的鉴定和抗原性检测[J].Chinese journal of Epidemiology,2003,24(10):917-919 |
| 中国莱姆病螺旋体PD91重组OspC的鉴定和抗原性检测 |
| Recombinant OspC identification and antigenicity detection from Borrelia burgdorferi PD91 in China |
| Received:January 20, 2003 |
| DOI： |
| KeyWord: 莱姆病螺旋体 外膜蛋白C 抗原性 |
| English Key Word: Borrelia burgdorferi Outer surface protein C Antigenicity |
| FundProject:科技部“十五”攻关课题资助项目 ( 2 0 0 1BA 70 5B0 7) |
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| Abstract: |
| 目的重组中国莱姆病螺旋体PD91外膜蛋白C(OspC)并在大肠埃希菌中表达,用于早期莱姆病诊断的研究。方法用聚合酶链反应(PCR)扩增出PD91外膜蛋白C基因,定向克隆到表达载体PET11D,构建重组质粒PET11DospC。用PCR、限制性内切酶分析及序列测定等方法鉴定重组质粒。用WesternBlot检测其抗原性。结果OspC基因被正确克隆到表达载体PET11D中。序列测定结果证实与国外已报道的序列存在一定的差异。OspC具有强抗原性。结论该项研究为国内莱姆病的早期特异性诊断的研究奠定了基础 |
| English Abstract: |
| Objective To recombine OspC gene from Borrelia burgdorferi PD91 of China and ex pressed it in E.coli for early diagnosis of Lyme disease.Methods The OspC gene was amplified from the genome of Borrelia burgdor feri PD91 strain by polymo rase chain reaction and recombined with plamid PET-11D.The recombinant plamid PET-11D-OspC was identified with PCR, restriction endo nuclease analysis and sequencing.The antig enicity was verified with Western Blot.Results OspC gene was cloned correctly into vector PET-11D.The resultant sequence was definitely different from the published sequence.The recombinant OspC seemed to have had strong antigenicity.Conclusion The findings laid basis for the studies on early diagnosis of Lyme disease. |
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