江苏省艾滋病病毒感染的分子流行病学研究

羊海涛; 邢辉; 贾成梅; 梁浩; 徐晓琴; 魏明; 李雷; 陈钊; 邵一鸣

Abstract

羊海涛,邢辉,贾成梅,梁浩,徐晓琴,魏明,李雷,陈钊,邵一鸣.江苏省艾滋病病毒感染的分子流行病学研究[J].Chinese journal of Epidemiology,2003,24(11):976-979

江苏省艾滋病病毒感染的分子流行病学研究

A molecular epidemiological study on the human immunodeficiency virus infection in Jiangsu province

Received:February 25, 2003

DOI：

KeyWord: 人类免疫缺陷病毒感染流行病学,分子

English Key Word: Human immunodeficiency virus Infection Epidemiology, molecular

FundProject:江苏省预防医学基金资助项目(99029)

Author Name	Affiliation
YANG Hai-tao	Jiang su Province Center for Diseases Prevention and Control, Nanjing 210009, China
XING Hui	中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫学实验室
JIA Cheng-mei	Jiang su Province Center for Diseases Prevention and Control, Nanjing 210009, China
LIAN G Hao	中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫学实验室
XU Xiao-qin	Jiang su Province Center for Diseases Prevention and Control, Nanjing 210009, China
WEI Ming	中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫学实验室
LI Lei	Jiang su Province Center for Diseases Prevention and Control, Nanjing 210009, China
CHEN Zhao	中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫学实验室
SHAO Yi-ming.	中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫学实验室

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Abstract:

目的了解江苏省不同人群中艾滋病病毒(HIV)各亚型毒株的流行情况和传播规律。方法收集HIV感染者及患者的流行病学资料;无菌采集HIV感染者或艾滋病(AIDS)患者抗凝全血标本5ml,提取前病毒DNA,用巢式聚合酶链反应(nestedPCR)扩增膜蛋白基因的C2～V3区,进行序列测定,鉴定病毒亚型。用威斯康星GCG软件进行共享序列、基因离散率的计算和毒株的聚类分析。结果截止2001年底,江苏省已发现HIV1中的A、B、C、D、E五种亚型和一个B亚型变种(B’)流行,C(占40.48%)和B’(38.10%)亚型为主要流行株;静脉吸毒感染人群中86.67%为C亚型,采供血和受血感染人群中91.67%为B’亚型,在性途径传播人群中所有六种亚型均有,且分布较为均一。结论有偿献血人群中B’亚型毒株由邻省传入,吸毒人群中C亚型毒株的传入主要与新疆籍流动人口的介入有关;多种亚型的并存说明江苏省存在着适宜AIDS流行的各种危险因素;提示今后在药物治疗、疫苗研制以及其他防制工作上将面临更大的困难

English Abstract:

Objective To identify subtypes of human immunodeficiency virus 1 ( HIV-1)strains and their distribution, infection sources, and the trends of HIV infection in Jiangsu province.Methods Anticongulated bloods from 46 infectedpersons were collected to separate previrus DNA. HIV-1 env genes were then amplified by nested-PCR and sequenced for their C2-V3 region so as to identify subtypes.The analysis of consensus sequence, genetic distance and phylogenetic tree were conducted with GCG software. Results By the end of 2001, there had been six subtypes of HIV-1 strains identified in Jiangsu province: A, B, B', C, D and E. The predominanTsubtypes were C( accounting for 40. 48%)and B' ( accounting for 38. 10%).Subtype Caccounted for 86. 67% among injecting DUs while subtype B' accounted for 91. 67% among commercial blood donors and receivers.Conclusion Subtype B' among commercial blood donorswas broughTto Jiangsu from neighboring provinces.The outbreak of HIV-1 infection amonglocal DUs was caused by subtype C from Xinjiang province. Findings from HIvaIDS molecular epidemiologic study suggesTthaTiTis challenging for Jiangsu to treaTpatients, apply vaccine, prevenTand control AIDS in the future.

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