| 吕红霞,方肇寅,谢华萍,唐景裕,胡海宽,郑丽舒,叶青,章青,ZHONGWei-min,Jiang XI.河北省卢龙县1999～2001年婴幼儿杯状病毒腹泻流行病学研究[J].Chinese journal of Epidemiology,2003,24(12):1118-1121 |
| 河北省卢龙县1999～2001年婴幼儿杯状病毒腹泻流行病学研究 |
| Epidemiological study of human caliciviruses among children with acute diarrhea in Lulong county, 1999-2001 |
| Received:November 20, 2002 |
| DOI： |
| KeyWord: 人类杯状病毒 序列分析 流行病学 |
| English Key Word: Human calicivirus Sequence analysis Epidemiology |
| FundProject:美国国立卫生研究所基金资助项目 (RO3TW01192 ) ;国家“863”课题资助项目 (2001AA212171) |
| Author Name | Affiliation | | Lv Hongxia | Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China | | Fang Zhaoyin | Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China | | Xie Huaping | Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China | | Tang Jingyu | 河北省卢龙县卫生防疫站 | | Hu Haikuan | 河北省卢龙县卫生防疫站 | | Zheng Lishu | Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China | | Ye Qing | 秦皇岛市卫生防站 | | Zhang Qing | Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China | | Zhong Weimin | Children's Hospital Medical Center, Cincinati, OH, U SA | | Jiang Xi | Children's Hospital Medical Center, Cincinati, OH, U SA |
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| Abstract: |
| 目的 研究河北省卢龙县5岁以下婴幼儿杯状病毒腹泻的流行特点。方法 应用酶联免疫吸附试验 (ELISA)和逆转录 聚合酶链反应 (RT PCR)方法检测人类杯状病毒 (HuCVs)。部分阳性标本的PCR产物经克隆测序,结合参考毒株相应的核苷酸序列进行进化分析。利用住院腹泻患者粪便样本中HuCVs的检出率,估计HuCVs腹泻住院率。结果 HuCVs检出率为 31.6 %,住院患者HuCVs阳性率为 17.5 %,主要分布在 3～17月龄婴幼儿,发病主要集中在冬季。 11株测序病毒之间的核苷酸序列同源性为 55.1%～ 100 %,均属于诺瓦克病毒 (NLV)GⅡ遗传组。卢龙县 2000年HuCVs流行株为NLVGⅡ 4和GⅡ 7,2 0 0 1年为NLVGⅡ 3和GⅡ 7。初步估计HuCVs腹泻患者住院率为 3.6‰。结论 河北省卢龙县婴幼儿中存在不同基因型杯状病毒感染,以NLVGⅡ组毒株为主,其疾病负担仅次于轮状病毒。 |
| English Abstract: |
| Objective ?? To investig ate the epidemiological character istus of human caliciv iruses(HuCVs) among childr en under 5 years of age with acute diarrhea and to estimate the disease burden in Lulong county. Methods ?? HuCVs were detected by enzyme linked immunosorbent assay (ELISA) andr ev erse transcr iption- polymer ase chain r eaction ( RT- PCR). Some PCR amplicons were cloned and sequenced. Phy logenetic tree was constructed for str ain char acterization. The r ate of HuCVs-attributed hospitalization w as est imated accor ding to the positiv e rate of HuCVs detection in fecal specimens collected from hospitalized diarrhea patients. Results?? Between July 1999 and June 2001, 708 fecal specimens were collected, of which 393 rotavirus- negative and 5 rotavirus- positive specimens w ere detected fo r HuCVs.Thirty- one point six percertage of fecal specimens from patients with diarrhea was HuCVs positive. Among inpatients, HuCVs posit ive rate was 17. 5%. HuCVs detection was mainly distributed in 3- 17 mouth- old children, in w int er. All 11 strains belong ed to NLV G ?? in w hich 6 strains GⅡ- 3, 2 strains GⅡ- 4 and 3 strains GⅡ- 7, and t hey shared 55. 1%- 100% nucleotide identity. NLV GⅡ- 4 and GⅡ- 7 w ere identifiedin 2000, w hile NLV GⅡ- 3 and GⅡ- 7 in 2001. The pr eliminar y estimate of HuCVs- attr ibuted hospitalization r ate was 3.6 %. Conclusion?? Human caliciviruses w ith differ ent genotypes circulated among children in Lulong county w ith GⅡNLVs were the prevalent strains. The disease burden of HuCVs was second to rotavirus |
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