吉林省长白山区斑点热立克次体自然疫源地调查

郝永建; 曹务春; 高淑萍; 张泮河; 赵秋敏; 杨红; 张习坦

Abstract

郝永建,曹务春,高淑萍,张泮河,赵秋敏,杨红,张习坦.吉林省长白山区斑点热立克次体自然疫源地调查[J].Chinese journal of Epidemiology,2003,24(12):1126-1128

吉林省长白山区斑点热立克次体自然疫源地调查

A new type of spotted fever group Rickttsiaes detected in the area of Changbai mountain, Jilin province

Received:October 21, 2002

DOI：

KeyWord: 蜱斑点热立克次体序列分析

English Key Word: Ticks Spotted fever group Rickttsiaes Sequence analy sis

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Author Name	Affiliation
Hao Yongjian	The Preventive Team of People's Liberation Army Second Artillery, Beijing 100071, China
Cao Wuchun	军事医学科学院微生物流行病学研究所
Gao Shuping	The Preventive Team of People's Liberation Army Second Artillery, Beijing 100071, China
Zhang Panhe	军事医学科学院微生物流行病学研究所
Zhao Qiumin	军事医学科学院微生物流行病学研究所
Yang Hong	军事医学科学院微生物流行病学研究所
Zhang Xitan	军事医学科学院微生物流行病学研究所

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Abstract:

目的了解吉林省长白山区蜱传斑点热的自然疫源地情况。方法利用立氏立克次体[相对分子质量 (Mr) 190× 10 ³ ]外膜蛋白A(R.rOmpA)基因序列设计引物,对 6 83只蜱类标本进行聚合酶链反应 (PCR)检测,并随机抽取一株森林革蜱阳性扩增产物进行克隆与序列测定。结果从森林革蜱和嗜群血蜱标本中检测出了斑点热立克次体DNA片段,阳性率分别为 53.81%和 7.41% ;所测序列与前苏联的DnS 14株的同源性为 100.00 %,与DnS 2 8和RpA 4的同源性均为99.00 % ;而与国内所检测的BJ 90、HLJ 054的同源性分别为 88.00 %和 86.00 %。结论长白山区存在斑点热的自然疫源地,存在与DnS 14株型别一致的斑点热立克次体,在中国系首次发现。

English Abstract:

Objective In order to find out the cur rent situa tio n of tick-borne spotted fever in the area of Changbai mountain, Jilin province Methods I n this study, a polymerase chain reactio n(PCR) method was dev elo ped with primers R.rOmpA 190.70p and R.rOmpA 190.701n designed on the basis of rOmpA gene, w hich is specific fo r ex amining spo tted fev er g roup Rickttsiaes (SFGR).Six hundred nig htythree ticks were tested and a positive PCR pro duct amplified from D.silv arum specimen (named JL-02) was cloned and sequenced.Results The SFGR DNA was detected from D.silvarum, Haemaphysalis concinna with the positive rates were 53.81% and 7.41 % respectively.Its nucleotide sequence of 587 bp rOmpA and deriv ed amino-acids show ed 100.00% similarity with nucleo tide sequence of DnS 14 and99.00 % with DnS 28 from the Former Soviet Union according to the result of BLUST and CLUSTAL, which w as differential from the DNA sequences of strains previously detected in China.Conclusion The natural focus of tick-borne spotted fever did exist in the area of Changbai mountain.The DNA sequence of SFGR was similar to that of DnS 14, which was first reported in China.

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