| 王定明,郝琴,蔡星和,万康林,王昭孝,陈建.贵州省莱姆病螺旋体的核糖体基因分型研究[J].Chinese journal of Epidemiology,2003,24(12):1129-1131 |
| 贵州省莱姆病螺旋体的核糖体基因分型研究 |
| Study on ribotyping of Lyme borreliosis spirochete in Guizhou province |
| Received:September 29, 2002 |
| DOI： |
| KeyWord: 莱姆病螺旋体 核糖体基因分型 流行病学 分子 |
| English Key Word: Lyme borreliosis spirochete Ribotyping Epidemiology molecular |
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| Abstract: |
| 目的 对从贵州省农耕区的鼠类动物分离到的 2 1株莱姆病螺旋体菌株进行分子流行病学研究。方法 应用聚合酶链反应 (PCR)从 2 1株莱姆病螺旋体分离株的全基因组DNA扩出 5S～2 3SrRNA基因间隔区,扩增产物用限制性片段长度多态性分析 (RFLP)和核酸序列分析。结果 贵州省 2 1株莱姆病螺旋体菌株可分为两个基因型 :Borreliavalaisiana(B.valaisiana) 2 0株,Borreliasp1株。结论 贵州省农耕区以B.valaisiana基因型为主,目前B.valaisiana对人的致病性已经得到证实。 |
| English Abstract: |
| Objective To define the main genotypes in Guizhou agricultural areas by molecular epidemiologic investig ation of 21 Borrelia burgdor feri sensu lato of Lyme disease spirochetes and to provide the scientific bases for formulating a preventive policy.Methods Polymerase chain reaction(PCR)technique w as used to amplify the 23S(rrl)-5S(rrf)intergenic spacer, and amplified products were analyzed by restrictio n fragment length polymo rphism(RFLP)and nucleotide sequencing.Results There were two genospecies in the strains:20 str ains belo ng to Borrelia v alaisiana, 1 strain is Borelia sp.Conclusion Borrelia valaisiana was the main geno ty pe in Guizhou agricultural areas.The harmness of B.valaisiana to human being has been co nfirmed.In order to efficiently prevent the harmness of ag ent to the people in Guizhou ag riculture areas, we should study the risk further |
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