乙型肝炎患者人类白细胞抗原-DRB1、-DQA1、-DQB1等位基因多态性分析

蒋业贵; 王宇明

Abstract

蒋业贵,王宇明.乙型肝炎患者人类白细胞抗原-DRB1、-DQA1、-DQB1等位基因多态性分析[J].Chinese journal of Epidemiology,2004,25(4):337-340

乙型肝炎患者人类白细胞抗原-DRB1、-DQA1、-DQB1等位基因多态性分析

Study on the polymorphisme of human leucocyte antigen-DRB1,-DQA1 and -DQB1 alleles in patients with hepatitis B

Received:February 07, 2003

DOI：

KeyWord: 乙型肝炎人类白细胞抗原遗传易感性

English Key Word: Hepatitis B Human leucocyte antigen Genetic susceptibility

FundProject:

Author Name	Affiliation
JIANG Ye-gui	400038 重庆,第三军医大学西南医院全军感染病研究所
WANG Yu-ming	400038 重庆,第三军医大学西南医院全军感染病研究所

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Abstract:

目的探讨人类白细胞抗原（HLA）-DRB1、-DQA1和-DQB1等位基因多态性与乙型肝炎（乙肝）之间的关系。方法采用聚合酶链反应/序列特异性引物（PCR/SSP）技术对52例慢性乙肝患者、30例急性乙肝患者和106名正常人的HLA-DRB1、-DQA1和DQB1等位基因多态性进行了分析。结果 HLA-DRB1*0301、-DQA1*0501和-DQB1*0301在慢性乙肝患者组的等位基因频率（17.31%、25.96%、35.58%）明显高于正常对照组（5.67%、13.36%、18.87%）,两者相比差异有显著性(X₁²=12.3068,P_c1=0.0074;X₂²=9.2002,P_c2=0.0157;X₃²=15.5938,P_c3=0.0075)。HLA-DRB1*1101/1104和-DQA1*0301在慢性乙肝患者组的等位基因频率（0.96%、14.42%）明显低于急性乙肝患者组（13.33%、30%）,两者相比差异有显著性(X₁²=11.9206,P_c1=0.0145;X₂²=8.7396,P_c2=0.0167)。结论 HLA-DRB1*0301、-DQA1*0501和-DQB1*0301与慢性乙肝患者的易感性密切相关,HLA-DRB1*1101/1104和-DQA1*0301与慢性乙肝的抗性密切相关,说明宿主的HLA-Ⅱ类基因是决定乙肝病毒感染转归的重要因素。

English Abstract:

Objective To investigate the association between the polymorphism of human leucocyte antigen(HLA)-DRB1,-DQA1 and -DQB1 alleles and viral hepatitis B. Methods HLA-DRB1,-DQA1 and -DQB1 alleles in 52 patients with chronic hepatitis B, 30 patients with acute hepatitis B and 106 normal control subjects were analysed, using the polymerase chain reaction/sequence specific primer (PCR/ SSP) technique. Results The allele frequencies of HLA-DRB1 * 0301, -DQA1 * 0501 and -DQB1 * 0301 in the chronic hepatitis B group (17.31%, 25.96%, 35.58%) were markedly higher than that in the normal control group (5.67%, 13.36%, 18.87%), with statistical significance (X_1~2=12.3068, P_(c1)= 0.0074; X_2~2=9.2002, P_(c2)=0.0157; X_3~2x=15.5938, P_(c3)=0.0075). The allele frequencies of HLA-DRB1 * 1101/1104 and -DQA1 * 0301 in the chronic hepatitis B group (0.96%,14.42%) were markedly lower than that in the acute hepatitis B group (13.33%,30%), with significant correlation between them (X_1~2= 11. 9206,P_(c1)=0.0145;X_2~2=8.7396, P_(c2)=0.0167). Conclusion HLA-DRB1 * 0301,-DQA1 * 0501 and -DQB1 * 0301 were closely associated with the susceptibility to chronic hepatitis B, while HLA-DRB1 * 1101/1104 and -DQA1 * 0301 closely associated with the resistance to chronic hepatitis B. These findings suggested that host HLA class Ⅱ gene was an important factor determining the outcome of HBV infection.

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