| 龚震宇,翁景清,赵芝雅,陈毓土,祝阿根,傅桂明,方春福,朱智勇,陈恩富,汪垂章,王玮,雷林海,王臻.肾综合征出血热疫苗免疫后血清学监测[J].Chinese journal of Epidemiology,2004,25(5):400-402 |
| 肾综合征出血热疫苗免疫后血清学监测 |
| Serological surveillance on hemorrhagic fever renal syndrome after vaccination |
| Received:March 28, 2003 |
| DOI： |
| KeyWord: 肾综合征出血热 灭活疫苗 血清学 监测 |
| English Key Word: Hemorrhagic fever renal syndrome Inactivated vaccine Serology Surveillace |
| FundProject:国家“九五”医学科技攻关课题资助项目(96-906-13) |
| Author Name | Affiliation | | Gong Zhen-yu | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China | | Weng Jing-qing | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China | | Zhao Zhi-ya | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China | | Chen Yu-tu | 龙游县疾病预防控制中心 | | Zhu A-gen | 龙游县疾病预防控制中心 | | Fu Gui-ming | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China | | Fang Chun-fu | 衢州市疾病预防控制中心 | | Zhu Zhi-yong | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China | | Chen En-fu | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China | | Wang Chui-zhang | 衢州市疾病预防控制中心 | | Wang Wei | 衢州市疾病预防控制中心 | | Lei Lin-hai | 龙游县疾病预防控制中心 | | Wang Zhen | Center for Disease Control and Prevention, Zhejiang Province, Hangzhou 310009, China |
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| Abstract: |
| 目的: 监测肾综合征出血热(HFRS)疫苗免疫接种人群血清抗体水平变化并评价疫苗血清学效果。方法: 以村为单位, 16～60岁健康者(删除既往患HFRS者、外出9个月及疫苗禁忌症者)为观察对象, 按接种和对照各半的原则随机分组, 接种组共10460人, 对照组16159人。采用间接免疫荧光法测特异性IgG荧光抗体, 用微量细胞病变中和试验测中和抗体。结果: 接种前血清抗体阴性者全程接种后2周荧光抗体阳转率为100.0% (67/67, 95% CI: 96.3～100.0), 中和抗体阳转率为44.4% (8/18, 95%CI:22.0～69.0), 几何平均滴度分别为72.1和4.6。1年后进行加强免疫, 加强免疫前、免疫后2周, 免疫后1年、1.5年、2年、3年和5年, 荧光抗体阳性率分别为28.6%、83.3%、75.0%、53.1%、22.6%、10.0%、55.0%; 中和抗体阳性率分别为14.8%、55.6%、35.0%、31.3%、26.0%、10.0%、50.0%。免疫(感染)增强反应需要进一步研究。结论: HFRS疫苗免疫效果良好,血清抗体持续时间较长。 |
| English Abstract: |
| Objective: To observe the serological and epidemiological efficacy of hemorrhagic fever renal syndrome(HFRS) vaccine in Zhejiang province. Methods: Immunofluorecent antibody assay and Mcro-CPE method were used to test specific IgG antibody and the titer of neutralizing antibody. Results: Two weeks after the injection of the third dose, the sero-conversion rates by both immunofluorecent antibody test(IgG) and neutralization test were 100.0% (67/67) (95% CI:96.3-100.0) and 44.4%(8/18) (95% CI 22.0-69.0) with geometric mean titers(GMTs) 72.1 and 4.6 respectively. The rates of seroconversion of immunofluorescent antibody by immunofluorescence antibody assay(IFA) were 28.6%, 83.3%, 75.0%, 53.1%, 22.6%, 10.0% and 55.0% before reinforcement, two weeks, one year, one year and a half years, two years, three years and five years after reinforcenment. The rates of neutralizing antibody seroconvversion by the Mcro-CPE method were found as 14.8%, 55.6%, 35.0%, 31.3%, 26.0%, 10.0% and 50.0% respectively. We found some antibody dependent immunization enhancement phenomenon among the inoculated population, but futher observation was needed. Conclusion: HFRS vaccine was immunologically effective and the duration of serous antibody last long. |
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