| 肖文珺,王明连,魏巍,王洁,赵建军,易滨,李劲松.SARS患者某定点收治医院空气样本中SARS-CoV及其RNA的检测[J].Chinese journal of Epidemiology,2004,25(10):882-885 |
| SARS患者某定点收治医院空气样本中SARS-CoV及其RNA的检测 |
| Detection of SARS-CoV and RNA on aerosol samples from SARS-patients admitted to hpital |
| Received:January 28, 2004 |
| DOI： |
| KeyWord: 严重急性呼吸综合征|SARS冠状病毒|空气|序列分析 |
| English Key Word: Severe acute respiratory syndrome|SARSCoV|Air|Sequence analysis |
| FundProject:全军非典型肺炎防治重大科技项目资助(03F015-02) |
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| Abstract: |
| 目的 了解SARS某定点收治医院空气中的SARS病毒污染情况及SARS病毒在空气中的分布和传播特性。方法 采用FA-2型空气微生物采样器在病房区及病房阳台连续采样。将采集到的空气样本洗脱后分别采用细胞分离和逆转录-聚合酶链反应分析,并对细胞分离阳性者作进一步的系列分析鉴定。结果 SARS患者某定点收治医院病房区及阳台空气样本中均有部分PCR结果阳性,SARS阳性率病房区为29%,阳台为20%;其中一份样品中分离出活性病原体,并稳定传代;经免疫荧光染色鉴定阳性,RT-PCR扩增产物的测序结果显示该病原体与已知SARS-CoV的同源性在98%以上。结论 SARS急性期后期与恢复期早期的患者仍然从呼吸道排毒,病毒在距传染源周围1m之内的空气中具有感染活性,在此范围之内存在气溶胶传播的潜在威胁。 |
| English Abstract: |
| Objective To assess the risk of aerosol transmission in severe acute respiratory syndrome (SARS) patients admitted to Hospital through testing the air samples. Methods Air samples were collected from 7 wards and 1 balcony of the Hospital，3 times a day for 3 continuous days, using bioaerosol sampler type FA-2. Bioaerosol particles were then washed down from the samples by serum-free Dulbecco's Modified Eagle Medium(DMEM) culture medium. Nested-reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify the N protein gene of the SARS associated coronavirus (SARS-CoV) from these washing solutions. The residual solutions were inoculated into prepared cell cultures to isolate live virus. The positive samples were then identified by indirect immunofluorescence assay and sequence analysis of the PCR products. Results Positive rates of RT-PCR test on air samples were 29.03%in the wards and 20.0% in balcony respectively. Results from sequential analysis showed that the homology of amplified cDNA fragments to previously known SARS-CoV stains was 98%.A strain of live pathogen was isolated from one of the 36 samples. The isolate could cause typical cytopathic effects, similar to those SARSCoV on Vero-E6 cells and the effects could be stably passed. Indirect immunofluorescence assay showed positive from serum of a SARS patient. Conclusion SARS-CoV existed in the air hospital, where SARS patients were admitted to, but the activity of SARS-CoV in air samples was rather low. SARS patients could still shed SARS-C}V even during the recovery phase. Potential possibility of aerosol transmission might exist within I meter square area around SARS patients. |
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