| 李关汉,陈志伟,陈铮,魏飞力,梅珊,黄耀新,张林琦,曹韵贞.中国部分地区HIV-1流行株基因型分布与母婴传播[J].Chinese journal of Epidemiology,2004,25(12):1013-1018 |
| 中国部分地区HIV-1流行株基因型分布与母婴传播 |
| Study On the distribution Of human inlmunodeflciency Virus-1 subtypes in different regiOns Of China and mothe-to-child transmission |
| Received:January 16, 2004 Revised:January 16, 2004 |
| DOI： |
| KeyWord: 人免疫缺陷病毒 母婴传播 亚型 |
| English Key Word: Human immunodeficiency virus Mother-tchiId tmnsmiSSion subtype |
| FundProject: |
| Author Name | Affiliation | E-mail | | LI Guan-han | National Center for Sexually Transmitted Disease and Acguired Immune Deficiency Syndrome Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China | | | CHEN Zhi-wei | Aamn Diamond AIDS Research Center, Rockefeller University,USA | | | CHEN Zheng | Aamn Diamond AIDS Research Center, Rockefeller University,USA | | | WEI Fei-li | National Center for Sexually Transmitted Disease and Acguired Immune Deficiency Syndrome Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China | | | MEI Shan | National Center for Sexually Transmitted Disease and Acguired Immune Deficiency Syndrome Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China | | | HUANG Yao-xin | Aamn Diamond AIDS Research Center, Rockefeller University,USA | | | ZHANG Lin-gi | Aamn Diamond AIDS Research Center, Rockefeller University,USA | | | CAO Yun-zhen | National Center for Sexually Transmitted Disease and Acguired Immune Deficiency Syndrome Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China | yzcao@163bj.Com |
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| Abstract: |
| 目的 了解中国部分地区艾滋病病毒(HIV)-1主要流行区的病毒基因型分布特征及其对母婴传播的影响。方法 通过巢式聚合酶链反应对来自全国11个省(区)的HIV-1阳性病例(包括母婴病例)的gag基因和env基因的部分区域进行扩增并测序,采用DNA分析软件进行系统树和距离等分析。结果 共完成gag基因p17测序60例,env基因C2-V4区测序69例。新疆自治区和河南地区的流行株均很单一,前者为C亚型,河南省及周边地区为泰国B亚型(B'),云南地区主要为C和E亚型,而在北京和上海地区有A、B、C、E等多种不同亚型。新疆的病毒株与云南地区的C亚型极为相似,来源相似。在32对母婴病例中,主要为B和C亚型,E亚型1例,未定型2例。B亚型母亲的母婴传播率(50.0%)较明显地高于C亚型(26.7%),但差异无统计学意义。结论 中国部分地区HIV-1的亚型分布具有明显的地域特性,亚型对母婴传播的影响尚不清楚。 |
| English Abstract: |
| Objective To study the distribution of human immunodeficiency virus(HIV)一1 genotypes in major prevalent regions of China and to mustrate the rdatianship between HIV—l subtypes and mothe卜t口child transmission in a retrospective cohort．Methods HIV一1 gag p17 and env C2一V4 region were amplified by nested—polymerase chain reaction(nPCR)and the sequences were obtained by sequencing gag nPCR products or clones of env gene．ResuIts 60 HIV一1 positive individuals were subject to t)，ping for gag p17 and 69 fOr env C2一V4 region．Single clade was only found in Henan(subtype B')and Xinjiang(subtype C)，and subtypes C and E were demonstrated in Yunnan．These regions represented most of the H11V一1 infections in China． Multiple subtypes(A，B，C，E，etc．)were found in Be巧ing and Shanghai，where HIV infections were still in low 1evel．The sequences of subtype C were less diversive in Xinjiang(p17：0．0192±0．0078，C2一V4：0．0455±0．0145)than in Yunnan(p17：0．0279±O．0102，C2一V4：O．0482±0．0171)，but aU of them clustered in“C”branch in phylogenetic trees．Trafficking of subtype C from Yunnan to Xinjiang was fOund but had already been reported by others．(bmpared to subtype C，subtype E was quite divergent(p17：0．0473±0．0105，C2一V4：O．1114±0．0112)in Yunnan，but no recombination was found in the C2一V4 region of env gene． Highe divergence of subtype B'was found in Henan and the peripheral provinces(p17：0．0381±0．0101，C2一V4：0．0691±0．0166)，which might be attributed to the early epidemics of HIV一1 in these areas(early 1990's)． In maternal—child cohort，subtypes B(7／21)，C(11／21)，E(1／21)and undefined types(2／21)were identified in non—transmitting HIV一1 positive mothers，while only subtype B(7／11)and C(4／11)appeared in transmitting HIV一1 positive mothers．The rate of transmission was 53．8%(7／1 3)in mothers infected with subtype B and 30．8%(4／13)in those infected with subtype C，but with no significant difference(P=0．196)．The imbalancing distribution of subtypes might be explained by the fact that transfusion or i11egal bl∞d would increased mother—to_child transmission on HIV．1 and moSt of mothers with clade B were infected by illegal b100d transfusion in this cohort． In addition，most of the maternal—child pair's sequences clustered in gag or env phylogenetic trees but onIy a few did disperse among the unrelated patients because children were older(≥4 yearS)．c帅cl璐ion The characteristics of HIV一1 clade's distributian differed over moSt parts of China but no difference was demonstrated between subtype B and C in mother-t0-child transmiSsion on HIV一1． |
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