三种蜱媒传染病在媒介蜱和鼠类中复合感染的研究

赵秋敏; 吴晓明; 张泮河; 李建民; 杨红; 魏茂提; 张习坦; 曹务春

Abstract

赵秋敏,吴晓明,张泮河,李建民,杨红,魏茂提,张习坦,曹务春.三种蜱媒传染病在媒介蜱和鼠类中复合感染的研究[J].Chinese journal of Epidemiology,2005,26(1):9-13

三种蜱媒传染病在媒介蜱和鼠类中复合感染的研究

Study on the coinfection of three tick2borne infectious diseases in China using polymerase chain reaction method

Received:February 02, 2004

DOI：

KeyWord: 蜱媒传染病复合感染埃立克体,人粒细胞埃立克体,查菲

English Key Word: Tick-borne disease Coinfection Human granulocytic ehrlichia Ehrlichia chaf f eensis

FundProject:国家自然科学基金资助项目(39970655)

Author Name	Affiliation
ZHAO Qiu-min	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
WU Xiao-ming	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
ZHANG Pan-he	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
LI Jian-min	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
YANG Hong	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
WEI Mao-ti	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
ZHANG Xi-tan	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China
CAO Wu-chun	Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing 100071, China

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Abstract:

目的了解中国部分地区埃立克体病和其他蜱媒传染病病原体的复合感染情况。方法运用聚合酶链反应方法对内蒙古自治区、黑龙江省、北京市的蜱和鼠类标本粒细胞埃立克体、莱姆病螺旋体、斑点热群立克次体的感染进行检测,对福建省的蜱和鼠脾脏标本中查菲埃立克体、斑点热立克次体及莱姆病螺旋体的感染情况进行检测。结果内蒙古采集的全沟硬蜱408只,人粒细胞埃立克体感染率6.8%,莱姆病螺旋体感染率7.8%,斑点热群立克次体感染率45.6%,其中粒细胞埃立克体与莱姆病螺旋体复合感染标本5份(5/408),粒细胞埃立克体与斑点热群立克次体复合感染标本1份(1/408)。黑龙江采集的46只全沟硬蜱中人粒细胞埃立克体感染率6.5%,莱姆病螺旋体感染率10.8%,斑点热群立克次体感染率34.8%,其中粒细胞埃立克体与莱姆病螺旋体复合感染标本1份(1/46)。北京市采集的蜱类标本922只,有2只检测到莱姆病螺旋体的DNA片段。福建省宁化地区采集的优势蜱种越原血蜱283组(659只)中,查菲埃立克体最小阳性率为3.8%(25组阳性),38份野鼠脾脏的阳性率为56.4%(21只阳性),检测出有2只(2/659)蜱同时感染了粒细胞埃立克体和斑点热群立克次体,野鼠的脾脏标本中,也发现有一只同时感染了查菲埃立克体和斑点热立克次体。结论人粒细胞埃立克体和莱姆病螺旋体复合感染以及查菲埃立克体和斑点热群立克次体复合感染可能在特定地区发生;其地区的蜱类、鼠类存在着极低的复合感染。

English Abstract:

Objective To study the existence of Ehrluichiosis ,lyme disease and tick2borne spotted fever coinfection in some areas in China. Methods Using polymerase chain reaction ( PCR ) , B . burgdorf eri sensu lato , spotted fever group ( SFG) Rickettsiae and human granulocytic ehrlichia (HGE) , Ehrlichia chaf f eensis ( EC) were detected in ticks and mouse samples collected from Inner Mogolia autonomous region , Heilongjiang province ,Beijing and Fujian province. Results 408 Ixodes persulcatus collected from Inner Mogolia autonomous region , HGE and B . burgdorf eri sensu lato and SFG Rickettsiae were detected positive , with rates as 6. 8 % ,7. 8 % ,45. 6 % , respectively. 5 (5/ 408) were coinfection with HGE and B . burgdorf eri sensu lato while 1 (1/ 408) was coinfection with HGE and SFG Rickettsiae. 46 Ixodes persulcatus collected from Helongjiang province were determined positive , with rates as 6. 5 % , 10. 8 % and 34. 8 % , respectively including 1 (1/ 46) coinfected with HGE and B . burgdorf eri sensu lato. 2 of 922 ticks collected from Beijing were detected positive with B . burgdorf eri sensu lato. Among 283 groups of Haemaphysalis yeni ticks (3/ group) and from 38 rodent samples collected from Ninghua county of Fujian province HCE and B . burgdorf eri sensu lato and SFG Rickettsiae were detected. Out of them , 25 groups were positive with EC and the minimal positive rate was 3. 8 % while 21 rodent samples were positive with EC with a positive rate of 56. 4 %. 2 ticks and 1 rodent sample were detected positive with EC and spotted fever group. Conclusion Coinfection of HGE and B . burgdorf eri sensu lato or spotted fever group Richi did exist in Ixodes persulcatus collected from Inner Mogolia autonomous region and Heilongjiang province. Coinfection of EC and spotted fever group Richi was found in the ticks and rodents collected from Fujian province.

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