内皮型一氧化氮合成酶基因G894T变异与早发冠心病的关系

贾崇奇; 宁艳; 刘同涛; 刘兆兰

Abstract

贾崇奇,宁艳,刘同涛,刘兆兰.内皮型一氧化氮合成酶基因G894T变异与早发冠心病的关系[J].Chinese journal of Epidemiology,2005,26(1):51-53

内皮型一氧化氮合成酶基因G894T变异与早发冠心病的关系

Association between G894T mutation in endothelial nitric oxide synthase gene and premature coronary heart disease

Received:April 26, 2004

DOI：

KeyWord: 冠心病一氧化氮合成酶,内皮型基因

English Key Word: Cor onary heart disease Nitr ic ox ide sy nthase, endo thelial Gene

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Author Name	Affiliation
JIA Chong-qi	Department of Epidemiology and Health Statistics,School of Public Health, Shandong University, Jinan 250012, China
NING Yan	Department of Epidemiology and Health Statistics,School of Public Health, Shandong University, Jinan 250012, China
LIU Tong-tao	山东大学齐鲁医院心内科, 济南 250012
LIU Zhao-lan	Department of Epidemiology and Health Statistics,School of Public Health, Shandong University, Jinan 250012, China

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Abstract:

目的探讨内皮型一氧化氮合成酶基因第7外显子G894T变异(Glu298Asp)与早发冠心病之间的关系。方法以医院为基础的病例对照研究,选择新诊断的冠心病患者为研究对象。男性55岁以前及女性65岁以前患冠心病为早发冠心病。以132例早发冠心病患者为病例组,172例迟发冠心病患者为对照组。运用PCR限制性片段长度多态性检测G894T变异。结果G894T变异基因型频率早发冠心病组(TT、GT、GG频率分别为6.06%、20.45%、73.48%)显著高于迟发冠心病组(TT、GT、GG频率分别为1.74%、11.63%、86.63%)(P=0.01)。T等位基因频率早发冠心病组(16.29%)也显著高于迟发冠心病组(7.56%)(P=0.001),OR=2.38,95%CI:1.38～4.16。在α=0.05显著性水平上,用多元逐步非条件logistic回归调整性别、吸烟、饮酒、超重后,G894T变异对早发冠心病仍具有显著影响(P=0.01),OR=2.25,95%CI:1.19～4.26。结论eNOS基因G894T变异可能是早发冠心病发病的重要危险因素之一。

English Abstract:

Objective ?? To assess the association betw een G894T ( Glu298Asp) mutation in ex on 7 of t he endo thelial nitr ic ox ide sy nthase gene and premature coronar y heart disease ( P??CHD) . Methods Hospital??based case??control study w as conducted. New ly??diagnosed CHD patients were recruited as study subjects. 132 CHD patients diagnosed at/ befor e age 55 for males and 65 for females w er e assigned to P??CHD case g roup with other 172 CHD patients as the control gr oup. Polymer ase chain reaction with Ban ?? r estriction enzyme digestion w as performed to detect the G894T mutation. Results ?? G894T mutant g enotypes in P??CHD group ( TT, GT and GG fr equencies were 6. 06%, 20. 45% and 73. 48%, r espectiv ely) were significant higher than those in control group ( TT, GT and GG frequencies were 1. 74%, 11. 63% and 86. 63%, respectively ) ( P= 0. 01) . Mutant T allele frequency in P??CHD g roup w as also sig nificantly hig her than that in control gr oup ( 16. 29% v ersus 7. 56%, P = 0. 001, OR = 2. 38, 95% CI : 1. 38??4. 16) . Stepwise multiple logistic regression analysis at 0. 05 significant level w ith sex , smoking, alcohol dr inking, and overw eig ht covariates indicated that G894T mutation also hav ing significant effect on P??CHD ( P= 0. 01, OR = 2. 25, 95% CI : 1. 19?? 4. 26) . Conclusion ?? This study sug gested that G894T mutation in endothelial nitr ic ox ide sy nthase gene might serve as a major risk factor to the pathogenesis of P??CHD in this study population.

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