SARS恢复期患者血液与排泄物中SARS-CoV RNA的检测

常昭瑞; 杨仁全; 王彦斌; 任丽丽; 王敏; 杨耀武; 郭丽; 晁彦公; 曲成毅; 王健伟; 洪涛

Abstract

常昭瑞,杨仁全,王彦斌,任丽丽,王敏,杨耀武,郭丽,晁彦公,曲成毅,王健伟,洪涛.SARS恢复期患者血液与排泄物中SARS-CoV RNA的检测[J].Chinese journal of Epidemiology,2005,26(3):201-203

SARS恢复期患者血液与排泄物中SARS-CoV RNA的检测

Study on the RNA of severe acute respiratory syndrome( SARS)??associated coronavirus in the blood and excretion of convalescent patients with SARS

Received:September 09, 2004

DOI：

KeyWord: SARS冠状病毒排泄物恢复期患者

English Key Word: Severe acute respiratory syndrome??associated coronavirus Excretion Convalescent patient

FundProject:国家“863”计划专项基金资助项目(2003AA208403)

Author Name	Affiliation	E-mail
CHANG Zhao-rui	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China
YANG Ren-quan	南京农业大学
WANG Yan-bin	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China
REN Li-li	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China
WANG Min	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China
YANG Yao-wu	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China
GUO Li	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China
CHAO Yan-gong	清华大学附属北京酒仙桥医院
QU Cheng-yi	山西医科大学公共卫生学院	qucy@public.ty.sx.cn
WANG Jian-wei	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China	wangjw28@vip.sina.com
HONG Tao	National I ns titute f or Vir al Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 100052, China

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Abstract:

目的　应用巢式逆转录聚合酶链反应(RT-PCR)检测严重急性呼吸综合征(SARS)恢复期患者血液与排泄物中的病毒 RNA。方法　对连续 3 次收集的 23 例确诊 SARS恢复期(病程≥21天)患者的血、尿、痰、粪便标本进行核酸提取,设计特异性内外引物,使用巢式RT PCR的方法进行病毒RNA检测。结果　通过巢式RT PCR,共检测到6份阳性标本,其中粪便标本中检测到 4 份,检出率为5.8%痰标本中检测到 2 份,检出率为2.9%。在尿液和血液标本中未检测到病毒 RNA。结论个别SARS恢复期患者排泄物中有SARS病毒RNA存在,因此对恢复期患者的排泄物应慎重处理,对其引起SARS的再次传播的危险性需要进一步评估。

English Abstract:

ObjectiveTo examine the RNA of severe acute respiratory syndrome??associated coronavirus( SARS-CoV) in the blood and excretion of convalescent patient with SARS for prevention and treatment of the disease.MethodsA total of 276 samples, including plasma, urine, feces and sputum,obtained from 23 conv alescent patients w ith SARS were studied at 3 t ime??points at least 21 days after the onset of symptoms. RNA was extracted and nested rev erse tr anscript ion??polymerase chain r eaction( RT-PCR) was carried out using SARS??CoV specific pr imers. Results?? Among the 276 samples,SARS-CoV RNA was detected in 6 cases( 38.8%) by nested RT PCR. The positive rates of SARS??CoV-RNA was 5.8% in feces and 2.9% in sputum samples but SARS-CoV RNA was not detectable in plasma and urine of all the cases. Conclusion?? The existence of SARS-CoV RNA in the excretion of some convalescent patients with SARS showed that the ex cret ion from these patients should be carefully treated whil there??transmission of SARS by which, should be further studied.

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