| 赵雅男,姜庆五,姜仁杰,沈进进,陈胤忠,汪华.苏北地区2003年病毒性脑膜炎爆发病原分离株Echo30的序列分析[J].Chinese journal of Epidemiology,2005,26(4):282-285 |
| 苏北地区2003年病毒性脑膜炎爆发病原分离株Echo30的序列分析 |
| Sequence analysis of Echovirus type 30 isolated from an aseptic meningitis outbreak in northern Jiangsu province in 2003 |
| Received:May 12, 2004 |
| DOI： |
| KeyWord: 肿瘤,结直肠 胰岛素样生长因子 胰岛素样生长因子结合蛋白 Meta分析 |
| English Key Word: Enterovirus Echovirus 30 Sequence analysis |
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| Abstract: |
| 目的测定2003年苏北地区无菌性脑膜炎爆发的病毒分离株部分基因序列,了解该流行株的分子生物学特点及遗传变异规律。方法随机选取3株分离的病毒株,用能特异性扩增肠道病毒VP1区3'段序列的两对引物012/011、040/011进行逆转录聚合酶链反应,扩增产物经凝胶纯化后测序。将序列输入GenBank用BLAST程序进行核苷酸和氨基酸序列比对;选取部分肠道病毒序列,经CLUSTALX1.83对齐后,在PHYLIP3.5c中构建进化树,了解分离毒株Echo30的进化关系。结果3株病毒测序结果均为Echo30型肠道病毒。进化树分析显示分离的Echo30毒株与国外1999年和2000年分离株的亲缘关系最近;但3株分离株自成一簇,与国外毒株存在地区差别。结论利用VP1区3'段序列可以快速正确区分肠道病毒分离株的型别,分离株与国外同型流行株在比较区段的遗传变异规律类似。 |
| English Abstract: |
| Objective To determine the partial sequence of virus strains causing an aseptic meningitis outbreak in northern part of Jiangsu province in 2003 and to compare them with the same serotype strains isolated in other countries to better understand its genetic characteristics and hereditary trend of development.Methods Virus RNA was amplified using two sets of specific enteroviral 3' half of VP1 primers 012/011 and 040/011.Polymerase chain reaction(PCR) products were purified and sequenced.BLAST program was then used to perform on nucleotide and amino acid pairwise-alignment with all available sequences in NCBI database. Phylogenetic trees were drawed to compare with other enteroviral sequences using PHYLIP software. Results Under BLAST program,three sequences we submitted to GenBank were identically inferred as echovirus type 30,which had been identified by neutralization test in previous study. Phylogenetic analysis demonstrated that strains isolated from this ourbreak were aggregated into a cluster,and the closest relationships with them were those isolated in 1999 and 2000.This phenomenon indicated that Echo30 from this outbreak was different from other strains in different epidemic area. Conclusion 3' half of VP1 sequence could be used to quickly identify the serotype of isolated enterovirus.Strains isolated from this outbreak had the similar hereditary developing trend comparing with Echo30 strains isolated from other countries. |
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