| 蒋汝刚.湖北省咸宁市学龄前儿童营养不良危险因素的病例对照研究[J].Chinese journal of Epidemiology,2005,26(5):360 |
| 湖北省咸宁市学龄前儿童营养不良危险因素的病例对照研究 |
| Survival and growth of Vibrio cholerae O139 inside Acanthamoeba ?? |
| Received:December 16, 2004 |
| DOI： |
| KeyWord: 霍乱弧菌 棘阿米巴 细胞内共生生物 |
| English Key Word: Vibrio cholerae Acanthamoeba Endosymbiont |
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| Abstract: |
| 营养不良威胁儿童的生存、生命质量和后续发育, 也关系到我国人口素质的提高. 本研究旨在了解湖北省咸宁市学龄前儿童营养不良的危险因素, 为有效防治儿童营养不良提供科学依据. |
| English Abstract: |
| ObjectiveTo study the surviv al and grow th of Vibrio choler ae inside the Acanthamoeba polyphage.MethodsSurvival and growth of Vibrocholerae O139, co-cultured with Acanthamoeba polyphaga, was observed inside the trophozoites and cysts, using Gram stain and electron microscope. ResultsViable O139 was observed inside the amoebal vacuoles in 24 hours. Vacuoles were filled with more bacteria along with the longer period of co-culture. The process of O139 infection with A moebae would include uptake, formation of O139 vacuole, multiplication, trophozoites lysed and expelunder electron microscopy. Some infected trophozo ites could subsequently encyst and the sur viving O139 could locate in the vesicles inside the cysts. ConclusionO139 might survive and multiply in the trophozoites and reside inside the cysts of Amoebae, suggesting that Acanthamoebae might serve as one of the environmental hosts of Vibro cholerae. |
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