| 刘翼,戴迎春,姚英民,李建栋,詹惠春,陈清,俞守义,聂军.广州市某医院儿童秋冬季腹泻诺瓦克样病毒感染的分子流行病学研究[J].Chinese journal of Epidemiology,2005,26(7):525-528 |
| 广州市某医院儿童秋冬季腹泻诺瓦克样病毒感染的分子流行病学研究 |
| Molecular epidemiology of diarrhea among children with Nor walk-like virus in a hospital of Guangzhou in Autumn and Winter |
| Received:July 19, 2004 |
| DOI： |
| KeyWord: 诺瓦克样病毒 腹泻 流行病学 分子 |
| English Key Word: Norwalk-like virus Diarrhea Epidemiology molecular |
| FundProject: |
| Author Name | Affiliation | E-mail | | LIU Yi | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | | | DAI Ying-chun | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | | | YAO Ying-min | 广州南方医院儿科, 广州 510515 | | | LI Jian-dong | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | | | ZHAN Hui-chun | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | | | CHEN Qing | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | | | YU Shou-yi | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | | | NIE Jun | Epidemiology Department of Southern Medical University, Guangzhou 510515, China | niejun@fimmu.com |
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| Abstract: |
| 目的了解广州市某医院2003年秋冬季儿童腹泻中诺瓦克样病毒(NLVs)感染的流行病学特点及病毒的基因型。方法结果收集的358份标本中检测出NLVs42份,阳性率11.73%。3岁以下的婴幼儿阳性40例,占95.24%,其中6月龄～1岁组与1～2岁组阳性率最高,分别占38.10%、35.71%。年龄最小者只有25天龄。4个月中,11月阳性率最高为17.27%,其次是2004年1月、2003年10、12月,阳性率分别为15.79%、8.11%、11.36%。抽取11份阳性标本进行测序分析,结果5份为GⅡ-3群,5份为GⅡ-4群,1份为GⅡ型,但尚不确定所属群。随机抽取20份轮状病毒阳性标本检测NLVs,未发现合并感染。结论广州市某医院儿童腹泻的散发与NLVs感染有关,并且存在着不同的基因型,流行优势株为GⅡ-3群与GⅡ-4群的病毒株。 |
| English Abstract: |
| Objective To explore the epidemiological characteristics of Norwalk-like virus(NLVs) infection in children with diarrhea and to study the genotype and predominant cluster at a hospital in Guangzhou city. Methods Fecal specimens from 358 children with acute gastroenteritis from October 2003 to January 2004 and information about the cases were collected. NLVs was detected from the specimens by reverse transcription-polymerase chain reaction(RT-PCR) and the PCR products were purified and sequenced. Results Forty-two positive specimens were detected from the 358 fecal specimen with a positive rate of 11.73%(42/358). Of these, 40 specimens were obtained from infants younger than 3 years of age. The youngest infant infected with NLVs in this study was only 25 days. The positive rate in November(17.27%) was the highest. Eleven positive PCR products were selected and sequenced. Nucleotide sequence analysis revealed that 11 strains all belong to genogroupⅡ(GⅡ), and of these, 5 strains belonged to GⅡ-3 cluster, with another 5 strains belonged to GⅡ-4 cluster. However, one strain with its cluster could not be determined. Conclusion NLVs served as one of the important pathogens causing sporadic acute gastroenteritis among children at a hospital in Guangzhou. The predominant strains were identified as GⅡ-3 and GⅡ-4 cluster. |
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