| 汪明,李艳,张平安,杨超,向萍霞,韦叶生,李晓艳,黄从新.心肌梗死患者细胞间黏附分子-1基因多态性研究[J].Chinese journal of Epidemiology,2005,26(9):702-706 |
| 心肌梗死患者细胞间黏附分子-1基因多态性研究 |
| Study on the intercellular adhesion molecule-1 polymorphisms in a Chinese population with myocardial infarction |
| Received:October 21, 2004 |
| DOI： |
| KeyWord: 心肌梗死 细胞间黏附分子 基因多态性 |
| English Key Word: Myocardial infarction Intercellular adhesion molecule Gene polymorphism |
| FundProject:湖北省自然科学基金资助项目(2003ABA183) |
| Author Name | Affiliation | E-mail | | WANG Ming | Department of Clinical Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | LI Yan | Department of Clinical Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China | yanlitf@hotmail.com | | ZHANG Ping-an | Department of Clinical Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | YANG Chao | Department of Clinical Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | XIANG Ping-xia | Department of Clinical Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China | | | WEI Ye-sheng | 广西壮族自治区右江民族医学院附属医院检验科 | | | LI Xiao-yan | 武汉大学人民医院心内科 | | | HUANG Cong-xin | 武汉大学人民医院心内科 | |
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| Abstract: |
| 目的 探讨汉族人群中细胞间黏附分子-1(ICAM-1)第4外显子+12959G/A、第6外显子+13848A/G基因多态性与心肌梗死(心梗)的相关性。方法 提取165例心梗患者(包括急性心梗和陈旧性心梗)和199名健康对照者的DNA,采用序列特异性引物扩增法和聚合酶链反应-限制性片段长度多态性技术检测ICAM-1+12959G/A、+13848A/G基因多态性,同时测定血脂及血清超敏C反应蛋白(hsCRP,采用定量酶联免疫吸附试验技术)水平。结果 湖北地区汉族人群中存在+13848A/G多态性,在心梗组中基因型分布为:AA型58.2%、AG型37.0%、GG型4.8%;对照组分别为45.7%、45.2%、9.0%,两组比较差异有统计学意义(P<0.05);基因型频率的相对风险分析发现,AA型患心梗的风险是GG型及AG型的1.651倍(OR=1.651,95%CI:1.089～2.504)。未检出+12959G/A多态性。结论 ICAM-1+13848A/G基因多态性与湖北地区心梗易感性有关,+ 13848A等位基因可能是心梗发病的重要易感基因。 |
| English Abstract: |
| Objective To investigate the association between intercellular adhesion molecule-1 (ICAM-1) gene + 12959G/A and + 13848A/G polymorphism and myocardial infarction (MI). Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) technology and PCR-restriction fragment length polymorphism(PCR-RFLP) were used for the detection of ICAM-1 genotypes in 165 patients with MI(including acute MI and old MI) and 199 healthy controls. Plasma lipid levels and hyper sensitive C reactive protein (quantitive ELISA method was used) levels were measured in all subjects. Results + 12959G/A polymorphism was not detected in our study. The frequencies of AA, AG and GG Genotypes of + 13848A/G were 58.2% and 45.7%,37.0% among patients and 45? 2%,4.8% and 9.0% among controls, respectively. There were statistically significant differences in the distributions of the genotype frequencies(P < 0.05) between two groups,and the relative risk suffered from MI of AA genotype was 1.651 times of the GG and GA genotype(OR = 1.651,95 % C/ ： 1 ? 089-2.504). Conclusion There was no + 12959G/A polymorphism found in Chinese people. The coding single nucleotide polymorphism + 13848A/G in the exon 6 of ICAM-l gene was associated to MI and the allele A might serve as a risk factor for MI in Chinese. |
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