| 张潇,郑英杰,王法弟,高眉扬,朱建福,姜庆五.中国南方某农村地区人与猪戊型肝炎病毒的检测和部分序列分析[J].Chinese journal of Epidemiology,2005,26(12):984-987 |
| 中国南方某农村地区人与猪戊型肝炎病毒的检测和部分序列分析 |
| Detection and analysis of partial sequences isolated from human and swine in rural area of southern China |
| Received:February 05, 2005 |
| DOI： |
| KeyWord: 戊型肝炎病毒 基因型 人兽共患性疾病 |
| English Key Word: Hepatitis E virus Genotype Zoonosis |
| FundProject:复旦大学青年科学基金(JKF201001) |
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| Abstract: |
| 目的调查中国南方某农村地区戊型肝炎病毒(HEV)人株与猪株的相关性。方法应用逆转录-巢式聚合酶链法(RT-nPCR)对一般人群中HEV-IgM阳性者、急性戊型肝炎患者和当地某养猪场的猪进行HEV RNA检测，并对HEV RNA阳性标本进行克隆测序和序列分析。结果 132份猪粪便标本中13份为HEV RNA阳性；26份lgM阳性一般人群血清标本中有4份HEV RNA阳性；4例急性戊型肝炎患者中有1例的血清和粪便标本为阳性。序列分析发现该地区HEV人株与猪株在ORE2部分区域的核苷酸序列同源性为89.3%～100.0%，这10株HEV序列与HEV Ⅰ型、Ⅱ型、Ⅲ型和Ⅳ型在同一区域的核苷酸序列的同源性分别为78.7%～84.7%、83.3%～85.3%、76.0%～80.0%和84.7%～95.3%。结论该地区人群及猪群中流行的HEV同属基因Ⅳ型。 |
| English Abstract: |
| Objective To study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.Methods Specimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers.The positive PCR products were cloned and sequenced.Results 13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA.Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3%-85.3%, 76.0%-80.0% and 84.7%-95.3% nucleotide sequence identity with HEV genotype Ⅰ, Ⅱ, Ⅲ and Ⅳ respectively in the region (nt6317-6466).Conclusion HEV circulating in humans and swine in the area belonged to genotype Ⅳ. |
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