干扰素γ基因内含子1+874位点多态性与乙型肝炎病毒感染关系的研究

张平安; 吴健民; 李艳

Abstract

张平安,吴健民,李艳.干扰素γ基因内含子1+874位点多态性与乙型肝炎病毒感染关系的研究[J].Chinese journal of Epidemiology,2006,27(1):41-43

干扰素γ基因内含子1+874位点多态性与乙型肝炎病毒感染关系的研究

Relationship between genetic polymorphisms of Interferon2gamma gene intron 1 + 874 site and susceptibilityof hepatitis B virus infection

Received:April 11, 2005

DOI：

KeyWord: 乙型肝炎病毒干扰素γ基因多态现象(遗传) 易感性

English Key Word: Hepatitis B virus Interferon-gamma gene Polymorphism(Genetics) Susceptibility

FundProject:

Author Name	Affiliation
Zhang Pingan	Department of Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China
Wu Jianmin	华中科技大学同济医学院附属协和医院检验科
Li Yan	Department of Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China

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Abstract:

目的研究干扰素γ(IFN-γ)基因内含子1+874位点多态性与乙型肝炎病毒(HBV)感染、转归的关系,探讨慢性HBV感染的遗传易感因素。方法采用序列特异性引物-聚合酶链反应(PCR-SSP)技术检测231例慢性HBV携带患者、165例自限性HBV感染者和135名正常对照者IFN-γ基因内含子1+874位点T/A单核苷酸多态性。结果慢性HBV感染组IFN-γ基因+874位点AA基因型频率(TT、TA、AA频率分别为9.1%、12.1%、78.8%)高于正常对照组(TT、TA、AA频率分别为12.6%、23.0%、64.4%)(χ2=9.60,P=0.008);而自限性HBV感染组(TT、TA、AA频率分别为13.9%、23.7%、62.4%)和对照组之间差异无显著性(χ2=0.16,P=0.92)。结论IFN-γ基因内含子1+874位点多态性与慢性HBV感染有关,该位点多态性可能在决定个体HBV感染遗传易感性方面有一定意义。

English Abstract:

ObjectiveTo explore the possible relationship between the polymorphisms of interferon-gamma ( IFN2γ) gene intron 1 at position + 874 and the susceptibility of hepatitis B virus infection. Methods IFN2γgene single nucleotide polymorphism(intron 1 at position + 874) were detected in 531 subjects including 231chronic carrier of hepatitis B virus (group CC), 165 individuals who spontaneously recovered from HBV infection(group SR) and 135 healthy controls, by polymerase chain reaction with sequence specific primers. ResultsSignificant difference of IFN2γ + 874 AA genotype frequency was found between group CC ( TT, TA and AA frequencies were 9. 1 %, 12. 1 % and 78. 8 %, respectively) and control group (TT, TA and AA frequencies were 12. 6 %, 23. 0 % and 64. 4 %, respectively) (χ2 = 9. 60, P = 0. 008), but no significant difference found between group SR(TT, TA and AA frequencies were 13. 9 %, 23. 7 % and 62. 4 %, respectively) and control group (χ2 =0. 16, P = 0. 92). Conclusion　There was association between IFN2γgene polymorphism(intron 1 at position +874) and chronic HBV infection. This study suggested the possibility that IFN2γ gene polymorphism(intron 1 at position + 874) might be important in determining an individual’s susceptibility in the development of chronic HBV infection.

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