唐山地区原发性高血压与Y染色体非重组区HindⅢ酶切位点多态性的研究

李庆祥; 袁慧; 吴寿岭; 慈维苹; 李楠; 郝冰; 朱小玲; 李洪芬; 颜红兵; 陈祚; 刘国仗

Abstract

李庆祥,袁慧,吴寿岭,慈维苹,李楠,郝冰,朱小玲,李洪芬,颜红兵,陈祚,刘国仗.唐山地区原发性高血压与Y染色体非重组区HindⅢ酶切位点多态性的研究[J].Chinese journal of Epidemiology,2006,27(4):359-361

唐山地区原发性高血压与Y染色体非重组区HindⅢ酶切位点多态性的研究

Study on polymorphic HindⅢ restriction site of the Y chromosome and essential hypertension in Tangshan district

Received:July 14, 2005 Revised:July 14, 2005

DOI：

KeyWord: 高血压 Y染色体遗传学

English Key Word: Hypertension Y chromosome Genetics

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Author Name	Affiliation
LI Qingxiang	首都医科大学附属北京安贞医院
YUAN Hui	首都医科大学附属北京安贞医院
WU Shouling	唐山开滦医院
CI Weiping	首都医科大学附属北京安贞医院
LI Nan	首都医科大学附属北京安贞医院
HAO Bing	唐山开滦医院
ZHU Xiaoling	首都医科大学附属北京安贞医院
LI Hongfen	唐山开滦医院
YAN Hongbing	首都医科大学附属北京安贞医院
CHEN Zuo	中国医学科学院中国协和医科大学阜外心血管病研究所
LIU Guozhang	中国医学科学院中国协和医科大学阜外心血管病研究所

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Abstract:

目的探讨Y染色体非重组区HindⅢ酶切位点多态性与唐山地区汉族人群原发性高血压的关系。方法入选男性研究对象412名,原发性高血压患者225例、正常对照人群187名。所有研究对象用常规方法提取白细胞DNA。采用多聚酶链反应结合限制性内切酶(HindⅢ)方法检测 Y染色体非重组区HindⅢ酶切位点多态性。结果对照组和原发性高血压组Y染色体HindⅢ酶切位点多态性各基因型的差异有统计学意义(P=0．007)。HindⅢ(+)基因型较HindⅢ(-)基因型收缩压、舒张压、脉压和平均动脉压均明显降低(P<0．05)。结论 Y染色体HindⅢ酶切位点多态性与唐山地区汉族人群原发性高血压有关,可能是唐山地区汉族人群原发性高血压的一个遗传标志。

English Abstract:

Objective Since males are at higher risk of cardiovascular diseases than females, the aim of the study was to examine whether there is an association between BP and a polymorphic Hind Ⅲ biallelic marker in nonrecombining region of Y chromosome in essential hypertension in Tangshan district in China. Methods In the study,225 patients with essential hypertension and 187 healthy people were enrolled into this study as control group. DNA was extracted from white blood cell. Segments of polymorphic Hind Ⅲ restriction site of the Y chromosome were amplified from DNA by polymerase chain reaction (PCR). PCR products were restricted with 10 U of Hind Ⅲ for a night at 37℃. The digested products were subjected to electrophoresis in 3% agarose gels, and stained with ethidium bromide. Results We amplified 178 controls (95.2%) and 216 essential hypertensive patients (96.0%) successfully. Hind Ⅲ(-) genotype was found in 45. 8 % of the men in essential hypertension and in 32.0% of the men in the controlled group. The Hind Ⅲ(-) genotype was significantly higher than that in the controls (x2 = 7.782, P = 0.007). However, the Hind Ⅲ(+) genotype was lower in SBP(133.16 mm Hg±21.60 mm Hg vs. 143.58 mm Hg±24.16 mm Hg,P0.001), DBP(82.82mm Hg±11.72 mm Hg vs. 86.82 mm Hg±12.65 mm Hg,P=0.001),pulse pressure(50.34 mm Hg±14.31 mm Hg vs. 56.76 mm Hg±14.20 mm Hg, P0.001) and mean arterial pressure(99.59 mmHg±14.19 mm Hg vs. 105.74 mm Hg±15.31 mm Hg,P0.001) than the Hind Ⅲ(-) genotype. Conclusion Polymorphic HindⅢ restriction site of the Y chromosome seemed to be associated with essential hypertension in Tangshan district in China.

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