| 张铁钢,贺雄,陈丽娟,和京果,杨洁,孙美平,邵祝军,陈志海,赵辉.北京地区2005年流行性脑脊髓膜炎病原学监测[J].Chinese journal of Epidemiology,2006,27(5):396-398 |
| 北京地区2005年流行性脑脊髓膜炎病原学监测 |
| Surveillance on pathogens of meningococal meningitis in Beijing,2005 |
| Received:March 06, 2006 |
| DOI： |
| KeyWord: 流行性脑脊髓膜炎 血清群 多位点序列分析 脉冲场凝胶电泳分析 |
| English Key Word: Menimeningitis Serogroup Multilocus sequence typing Pulsed-field gel electrophoresis |
| FundProject: |
| Author Name | Affiliation | | ZHANG Tie-gang | Institute of Immunization and Prevention of Beijing Center for Disease Prevention and Control, Beijing 100013, China | | HE Xiong | Institute of Immunization and Prevention of Beijing Center for Disease Prevention and Control, Beijing 100013, China | | CHEN Li-juan | Institute of Immunization and Prevention of Beijing Center for Disease Prevention and Control, Beijing 100013, China | | HE Jing-guo | Institute of Immunization and Prevention of Beijing Center for Disease Prevention and Control, Beijing 100013, China | | YANG Jie | Institute of Immunization and Prevention of Beijing Center for Disease Prevention and Control, Beijing 100013, China | | SUN Mei-ping | Institute of Immunization and Prevention of Beijing Center for Disease Prevention and Control, Beijing 100013, China | | SHAO Zhu-jun | 中国疾病预防控制中心传染病预防控制所 | | CHEN Zhi-hai | 北京地坛医院感染中心一科 | | ZHAO Hui | 北京地坛医院检验科 |
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| Abstract: |
| 目的对2005年北京地区流行性脑脊髓膜炎(流脑)进行病原学监测。方法对临床流脑病例的血液、脑脊液标本进行特异性核酸片段检测,对鉴定阳性的菌株进行脉冲场凝胶电泳 (PFGE)和多位点序列(MIST)分析。结果在7份血液和5份脑脊液标本中检测到脑膜炎奈瑟菌的核酸片段。共分离到105株脑膜炎奈瑟菌。A群和C群菌株的群内菌株PFGE图谱无差异条带。 MLST分析结果表明北京地区A群脑膜炎奈瑟菌的序列类型主要是ST7,而C群主要是ST4821。结论北京地区目前流脑病例主要由序列类型为ST7的A群和ST4821的C群脑膜炎奈瑟菌引起。 |
| English Abstract: |
| Objective To study the pathogens of meningococcal meningitis (MM) in Beijing,2005.Methods Blood and cerebrospinal fluid specimens from MM patients were detected by polymerase chain reaction. Bacterial strains were analyzed by pulsed-field gel electrophoresis and multilocus sequence typing. Results 7 of the blood and 5 of cerebrospinal fluid specimens showed positive results. 105 of the Neisseria meningitides strains were isolated from the specimens of patients, close contacts and healthy carriers. Serogroup A and C Neisseria meningitides strains shared the same patterns of pulsed-fieldgel electrophoresis, respectively. The sequence type of serogroup A Neisseria meningitides belonged to ST7 while the sequence type of serogroup C Neisseria meningitides belonged to ST4821.Conclusion Patients suffered from meningococcal meningitis were caused by serogroup A ( ST7)and C(ST4821)Neisseria meningitides in Beijing, 2005. |
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