| 魏建春,张恩民,马凤琴,张建中.中国炭疽芽胞杆菌致死因子基因中单核苷酸多态性位点分析[J].Chinese journal of Epidemiology,2006,27(7):604-606 |
| 中国炭疽芽胞杆菌致死因子基因中单核苷酸多态性位点分析 |
| Analysis on single nucleotide polymorphism site in lef gene of Bacillus anthracis in the China isolates |
| Received:March 22, 2006 |
| DOI： |
| KeyWord: 炭疽芽胞杆菌 单核苷酸多态性 致死因子 |
| English Key Word: Bacillus anthracis Single nucleotide polymorphism Lethal factor |
| FundProject: |
| Author Name | Affiliation | E-mail | | WEI Jian-chun | National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | ZHANG En-min | National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | MA Feng-qin | National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | | | ZHANG Jian-zhong | National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China | Zhangjz@chinacdc.cn |
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| Abstract: |
| 目的分析中国炭疽芽胞杆菌致死因子基因中的单核苷酸多态性(SNP)位点特征。方法选择18株炭疽芽胞杆菌,采用PCR方法扩增炭疽芽胞杆菌致死因子基因、测序,并进行SNP位点分析。结果实验中所用中国炭疽芽胞杆菌致死因子基因中第895位核苷酸均为G,与国外菌株不同。中国不同地区(包括新疆、广西、北京、内蒙古等地区)、不同年代和不同来源(包括土壤、牛、羊、骡、狗、患者等)分离的菌株,具有相同的SNP位点特征。结论致死因子基因中第895位核苷酸均为G的SNP位点特征很可能是中国炭疽芽胞杆菌的共同特征。 |
| English Abstract: |
| Objective To analyze the characters of a single nucleotide polymorphism(SNP) site in the lethal factor gene(lef) of Bacillus anthracis isolated from China. Methods 18 Bacillus anthracis isolates were selected and the lef genes were amplified by PCR method, then the PCR products were sequenced and the analysis of SNP site of the lef genes was performed. Results The 895th nucleotide of lef gene of all tested Chinese isolates appeared to be G but T was found in some overseas strains. The characteristic of SNP site was identical in all strains isolated from different regions (included Xinjiang, Guangxi, Beijing, Inner Mongolia) at different times or sources (included soil, cow, goat, mule, dog, patient, etc) in China.Conclusion The 895th SNP site of lef gene might have shared the same characters of Chinese Bacillus anthracis strains. |
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