中国六省致病小肠结肠炎耶尔森菌的脉冲场凝胶电泳分析

金东; 崔志刚; 肖玉春; 王鑫; 顾锋; 夏胜利; 胡万富; 杨晋川; 汪华; 顾玲; 徐建国; 阚飙; 景怀琦

Abstract

金东,崔志刚,肖玉春,王鑫,顾锋,夏胜利,胡万富,杨晋川,汪华,顾玲,徐建国,阚飙,景怀琦.中国六省致病小肠结肠炎耶尔森菌的脉冲场凝胶电泳分析[J].Chinese journal of Epidemiology,2006,27(8):677-680

中国六省致病小肠结肠炎耶尔森菌的脉冲场凝胶电泳分析

Molecular typing of the pathogenic Yersinia enterocolitica strains with pulsed field gel electrophoresis isolated in China

Received:January 26, 2006

DOI：

KeyWord: 小肠结肠炎耶尔森菌脉冲场凝胶电泳分子分型

English Key Word: Yersinia enterocolitica Pulsed field gel electrophoresis Molecular typing

FundProject:国家“十五”科技攻关计划资助项目(2003BA712A05-04)

Author Name	Affiliation	E-mail
JIN Dong	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China
CUI Zhi-gang	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China
XIAO Yu-chun	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China
WANG Xin	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China
GU Feng	吉林省地方病第一防治研究所
XIA Sheng-li	河南省疾病预防控制中心
HU Wan-fu	安徽省疾病预防控制中心
YANG Jin-chuan	,江苏省徐州市疾病预防控制中心
WANG Hua	江苏省疾病预防控制中心
GU Ling	江苏省疾病预防控制中心
XU Jian-guo	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China	jinghuaiqi@icdc.cn
KAN Biao	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China
JING Huai-qi	National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China

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Abstract:

目的对中国致病小肠结肠炎耶尔森菌分离株进行脉冲场凝胶电泳分析。方法参照美国疾病预防控制中心PulseNet实验方法对中国6省165株致病小肠结肠炎耶尔森菌进行脉冲场凝胶电泳分析,使用BioNumerics软件进行聚类分析,并按照PulseNet命名原则对带型进行命名。结果将114株O:3血清型小肠结肠炎耶尔森菌分成25个带型;K6GN11C30012(50株)、K6GN11C30015 (19株)及K6GN11C30016(10株)是其主要带型,三个主要带型之间聚类相似性很高。将51株O:9血清型小肠结肠炎耶尔森菌分为14个带型;其中K6GN11C90004(22株)与K6GN11C90010(13株)是其主要带型,K6GN11C90004与K6GN11C90010带型之间有一定的差异。O:3与O:9血清型的主要带型之间有明显的差异。结论O:3血清型的小肠结肠炎耶尔森菌可能起源于一个克隆系,且在不同地区和年代变异很小;O:9血清型的小肠结肠炎耶尔森菌可能起源于两个不同的克隆系,且各自有一定的变异。O:3与O:9血清型的小肠结肠炎耶尔森菌亲缘关系较远。

English Abstract:

Objective To investigate the epidemiological and molecular typing features of the pathogenic Yersinia enterocolitica strains isolated in China，using pulsed field gel electrophoresis ( PFGE ) and standardized PFGE method as well as typing database of 1'ersinia enterocoliticu.Methods PFUE analysis was performed as Laboratory Directions for molecular subtyping of Salmonella by PFGE (PulseNet,USA) with some modifications and the results of PFGE were analyzed by BioNumerics soft (Version 4.0，Applied Maths BVBA, Belium ).Results 114 0:3 1'ersiniu enterocolitica strains were typed by 2S patterns to have found that K6GN11C30012(50 strains)，K6GN11C30015(19 strains ) and K6GN11C30016(10 strains) were the major patterns. K6GN11C30012 had 92.2% cluster similarity with K6GN 11 C30009-K6GN 11 C30023.This clone included 91.230/v strains of 114 0:3 1'ersinia enterocoliticu strains. 51 0:9 Yersinia enterocolitica strains were typed by 14 patterns; K6GN11C90004 (22 strains) and K6GN11C90010(13 strains)were the major patterns. K6GN11C90004 had 81.8%cluster similarity with K6GN11C90010 patterns. The major patterns of 0:3 and 0:9 serotypes were quite different.Conclusion 0:3 Yersinia enterexolitica strains might originate from the same clone and had very few variation in different years and provinces but 0:9 Yersinia enterocolitica strains from two different clones with some changes.

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