| 徐金水,王蓓,王长娴,郭海健,糜祖煌,凌健华.淋病奈瑟菌对氟喹诺酮类药物的耐药性与gyrA和parC基因突变关系的研究[J].Chinese journal of Epidemiology,2006,27(8):702-704 |
| 淋病奈瑟菌对氟喹诺酮类药物的耐药性与gyrA和parC基因突变关系的研究 |
| Study on Fluoroquinolone resistance and the relationship between resistance and mutations of gyrA and parC in Neisseria gonorrhoeae |
| Received:January 12, 2006 |
| DOI： |
| KeyWord: 淋病奈瑟菌 环丙沙星 耐药性 gyrA基因 parC基因 |
| English Key Word: Neisseria gonorrhoeae Ciprofloxacin Drug resistance gyrAgenes parC genes |
| FundProject:江苏省卫生厅预防基金资助项目(Y200408) |
| Author Name | Affiliation | E-mail | | XU Jin-shui | Department of Epidemiology, Public Health School of Southeast University, Nanjing 210009,China | | | WANG Bei | Department of Epidemiology, Public Health School of Southeast University, Nanjing 210009,China | wangbeixb@seu.edu.cn | | WANG Chang-xian | Department of Epidemiology, Public Health School of Southeast University, Nanjing 210009,China | | | GUO Hai-jian | Department of Epidemiology, Public Health School of Southeast University, Nanjing 210009,China | | | MI Zu-huang | 无锡市克隆遗传技术研究所
| | | LING Jian-hua | 香港中文大学微生物学系 | |
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| Abstract: |
| 目的探讨江苏省淋球菌对氟喹诺酮的耐药状况、耐药基因突变型的分布情况及二者之间的关系。方法采用琼脂稀释法检测淋球菌氟喹诺酮类药物(环丙沙星、左氧氟沙星)的最小抑菌浓度(MIC),采用PCR法扩增含有喹诺酮耐药决定区的gyrA和parC基因片段,并进行测序分析。结果根据所测MIC,本研究分离的95株淋球菌对环丙沙星100％的耐药;通过测序分析,在54株淋球菌中检测到gyrA和parC的18种突变形式,parC基因突变点越多,MIC相对也较高。结论parC基因的突变导致淋球菌对喹诺酮类药物的高水平耐药。 |
| English Abstract: |
| Objective To study the phenotypic and genotypic resistance to Fluoroquinolones in IVeisseria gonorrhoeae(NG) isolated in Jiangsu province of China.Methods In-vitro,susceptibility testing of ciprofloxacin and levofloxacin against ninety-five clinical isolates were determined by agar dilution method. Detection of mutation in the gyrA and parC genes was performed by polymerase chain reaction (PCR ) assay and sequence analysis.Results The clinical isolates demonstrated 100 % resistance to ciprofloxacin. Based on gyrA and parC mutations, 18 types could be categorized among the 54 isolates. Based on the same gyrA mutations.isolates with high MIC appeared to have had more mutations in parC gene.Conclusion The status of resistance to ciprofloxacin in NG was quite serious, and ciprofloxacin treatment for the treatment of NG infections in Jiangsu province should not be recommended. The results from this study suggested that mutations in the parC gene had contributed to the development of high Fluoroquinolone resistance in NG. |
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