| 窦亚玲,程歆琦,李永哲,韩建华,倪安平.乙型肝炎病毒DNA定量值与HBeAg、抗-HBe的相关性分析[J].Chinese journal of Epidemiology,2006,27(8):709-711 |
| 乙型肝炎病毒DNA定量值与HBeAg、抗-HBe的相关性分析 |
| Study on the correlation among quantification of HBV-DNA and HBeAg, anti-HBe in hepatitis B carriers |
| Received:January 05, 2006 |
| DOI： |
| KeyWord: 乙型肝炎病毒DNA 乙型肝炎e抗原 乙型肝炎e抗体 |
| English Key Word: Hepatitis B virus DNA HBeAg Anti-Hbe |
| FundProject: |
| Author Name | Affiliation | | DOU Ya-ling | Department of Clinical Labs, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730,China | | CHENG Xin-qi | Department of Clinical Labs, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730,China | | LI Yong-zhe | Department of Clinical Labs, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730,China | | HAN Jian-hua | Department of Clinical Labs, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730,China | | NI An-ping | Department of Clinical Labs, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730,China |
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| Abstract: |
| 目的探讨乙型肝炎(乙肝)病毒DNA的拷贝数(HBV DNA)与HBeAg、抗-HBe的相关性。方法对883例乙肝患者采用美国雅培公司AXSYM全自动酶免疫分析仪及配套试剂,进行微粒子酶免分析(MEIA)法检测HBeAg、抗-HBe和Roche COBAS AMPLICOR定量PCR仪及COBAS AMPLICOR HBV MONITORTM试剂盒内标法检测患者的HBV DNA,并进行相关性分析。结果(1)HBV DNA与HBeAg呈线性正相关(r=0．505,P<0．01 Mean:HBV DNA:7．12×1012拷贝／ml; HBehg:218．31 S／CO)。DNA含量104拷贝／ml对应HBeAg:104 S／CO;DNA含量在105-108拷贝／ml,HBeAg:112 S／CO;DNA含量109-1015拷贝／ml,HBeAg:252S／CO。(2)HBV DNA与抗-HBe无线性正相关(r=-0．052,P=0．477>0．05 Mean:HBV DNA 8．0×1010拷贝／ml,抗-HBe:0．18 S／CO)。结论HBV DNA与HBeAg呈线性正相关,HBeAg>100 S／CO,HBV DNA>104拷贝／ml,病毒复制活跃。HBV DNA与抗-HBe无线性正相关性,HBeAg阴性、抗-HBe阳性,病毒复制有所下降,但病毒载量仍较高,不可忽略其传染性。 |
| English Abstract: |
| Objective To better understand the duplication of hepatitis B virus (HBV) in order to improve clinical diagnoses and treatments via quantitative measurement of HBV-DNA and comparison of correlation of HBV-DNA with HBeAg and anti-HBe.Methods For 883 hepatitis B patients with positive HBsAg, HBV-DNA was measured by COBAS AMPLICOR HBV MONITORT"' reagent and COBAS AMPLICOR quantitative PCR instrument. Microparticle enzyme immunoassay analysis (MEIA) was then carried out with fully automatic enzyme immunoassay analysis instrument made by Abbott Axsym from the U. S. to measure HBeAg and anti-HBe. Correlation was analysed by SPSS.Results(1)Positive correlation between 690 HBV-DNA positive and HBeAg positive with r=0.505(P< 0.01)was found with mean values as:HBV-DNA:7.12X 10'z copieslml;HBeAg:218.31 SICO. HBV-DNA:100 copieslml, HBeAg: 104 S/CO; HBV-DNA: 10，-10" copieslml, HBeAg: 112 SICO; HBV-DNA: 10，-10'5 copieslml, HBeAg:252 S/CO.(2)No correlation was found between 193 HBV-DNA and anti-HBe+with ;=-0.052(P=0.477 > 0.05)with Mean: HBV-DNA: 8.0 X 10'0 copieslml anti-HBe: 0.18 SICO.Conclusion HBV-DNA and HBeAg appeared to have had linear correlation, showing that HBeAg> 100 SICO,HBV-DNA> 100 copies/ml and hepatitis B virus were reproduced. However,HBV-DNA did not show linear correlation with anti-HBe as HBeAg negative and anti-HBe positive, the level of hepatitis B viral replication decrease slightly. But the virus load is still high. Infectivity can not neglect. |
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