| 李宁,战淑慧,宣世英,强新.原发性肝癌组织中螺杆菌感染的研究[J].Chinese journal of Epidemiology,2006,27(10):894-896 |
| 原发性肝癌组织中螺杆菌感染的研究 |
| Study on Helicobacter infection in liver tissue from hepatocellular carcinoma |
| Received:December 14, 2005 |
| DOI： |
| KeyWord: 螺杆菌 肝肿瘤 原发性 原位杂交 |
| English Key Word: Helicobacter Hepatocellular carcinoma Situ hybridization |
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| Abstract: |
| 目的研究螺杆菌感染与原发性肝癌(HCC)的关系。方法选取34例HCC患者的肝癌组织为研究对象(实验组),20例非肝癌手术患者肝组织作对照组。将两组标本制成病理切片,用于螺杆菌属的cDNA-mRNA原位杂交实验。通过定位测定来评价肝组织螺杆菌感染与HCC的相关性。结果利用原位杂交技术检测实验组和对照组标本螺杆菌属16S rRNA-mRNA,实验组有22例阳性,对照组皆为阴性(P＜0．01)。进一步用幽门螺杆菌(Hp)和肝螺杆菌(Hh)的cDNA探针进行原位杂交,Hp探针杂交结果与螺杆菌属探针杂交结果相符,阳性率为64．71%,而Hh探针杂交结果为阴性。结论HCC组织中存在Hp感染。 |
| English Abstract: |
| Objective To investigate the relationship between Helicobacter species and hepatocellular carcinoma(HCC). Methods Liver samples resected during operation from 34 patients with HCC diagnosed by histopathology and 20 without primary liver carcinoma as controls were studied. The two groups of sample were cranked out pathologic slice for in situ hybridization of Helicobacter, Helicobacter pylori and Helicobacter hepaticus. Qualitative and quantitative studies were used to assess the correlation of liver tissue Helicobacter infection with HCC. Results 64.71%(22/34) of the samples of HCC showed positive for Helicobacter specific 16S rRNA-mRNA gene by in situ hybridization, while none was positive in controls(P0.01).Conclusion Helicobacter pylori were found in the liver of patients with HCC. |
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