黑龙江省东部边境口岸莱姆病生物媒介调查及病原分型研究

何浩; 郝琴; 呼满霞; 侯学霞; 范东晖; 耿震; 张健; 张少华; 郑超; 吴永生; 王传松; 崔文富; 万康林; 杨丽炜

Abstract

何浩,郝琴,呼满霞,侯学霞,范东晖,耿震,张健,张少华,郑超,吴永生,王传松,崔文富,万康林,杨丽炜.黑龙江省东部边境口岸莱姆病生物媒介调查及病原分型研究[J].Chinese journal of Epidemiology,2007,28(1):70-73

黑龙江省东部边境口岸莱姆病生物媒介调查及病原分型研究

Investigation on the vectors of Borrelia burgdorferi and on the identification of the isolates along China-Russia boner in Eastern Heilongjiang province,China

Received:July 26, 2006

DOI：

KeyWord: 莱姆病蜱伯氏疏螺旋体基因分型

English Key Word: Lyme disease Tick Borrelia burgdorferi sensu lato Genotype

FundProject:国家“十五”科技攻关项目(2001BA705807)

Author Name	Affiliation	E-mail
HE Hao	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
HAO Qin	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
HU Manxia	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
HOU Xuexia	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
FAN Donghui	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
GENG Zhen	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室
ZHANG Jian	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
ZHANG Shaohua	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
ZHENG Chao	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
WU Yongsheng	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
WANG Chuansong	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
CUI Wenfu	Suifenhe Entrp-Exit Inspection and Quarantine Bureau, Suifenhe 157300, China
WAN Kanglin	中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室	wankanglin@icdc.cn
YANG Liwei	黑龙江出入境检验检疫局(杨丽炜)	yan91w@hUciq.gov.cn

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Abstract:

目的调查黑龙江省东部中俄边境口岸蜱的种类、分布、携带莱姆病螺旋体情况及病原基因型。方法捕蜱采用布旗法；蜱带莱姆病螺旋体率采用直接荧光法检查；病原体分离培养采用BsKⅡ培养基，对分离到的螺旋体用单克隆抗体(H5。、地。，。、H9，：。)、sD$PA(沲蛋白图谱分析、PCR—RFLP分析以及基因测序等方法进行表型和基因分型研究。结果黑龙江省东部7个中俄边境口岸蜱的种类为1科3属4种，即硬蜱科硬蜱属全沟硬蜱，革蜱属森林革蜱，血蜱属嗜群血蜱和日本血蜱。不同生态环境中蜱的分布不同，各口岸优势蜱种不同。全沟硬蜱带莱姆病螺旋体率较高，平均为31．4％，森林革蜱和嗜群血蜱的带菌率较低，平均为2．2％和3．8％，日本血蜱中未检出阳性者。首次在中俄边境口岸——东宁、同江采集的全沟硬蜱中分离到伯氏疏螺旋体并鉴定为B．舭r而ii基因型。结论黑龙江省东部边境口岸为莱姆病的自然疫源地。

English Abstract:

Objective To explore the fact that the east border of Heilongjiang province had been alyme disease natural focus, we investigated the species and distribution of ticks and isolated bacteria fromticks and identified genomic species of Borrelia burdorferi sensu lato. This study provided evidence forprevention and control of Iyme disease. Methods Ticks were caught by flagging method and Directimmunofluorescence method was used to detect the rate of bacteria borne by the tick. BSK Ⅱ culturemedium was used to isolate the agent and印ecific McAbs were used to identify the bacteria. SDS-PAGEprotein profile and PCR-RFLP method were also used to identify the species of Spirochetes. Results Ticks,collected from China-Russia border of east Heilongjiang province were classified including Ixodes persulcatusSchulze, Dermacentor sivarum Olener, Haemaphysalis concinna Kock,and Haema灿ysalis japonica Kock.We found that the distributon of ticks was different under different circumstances and the predominantspecies were also different in different ports. The rate of bacteria borne by Ixodes persulaatus Schulze was31.4 %, by Dermacenror sivarum Olener and Haemaphysalis concinna Kock were 2.296 and 3.8 96，respectively. However,it was negative for Haemaphysalis japonica Kock. Spirochetes isolated from Ixalespersulcatus Schulae were collected from Dongning and Tongjiang while Genomic species of Spirochetes,isolated from ticks of the border belonged to B. garinii. Conclusion All the Results showed that the eastborder of Heilongjiang province was the natural focus of lyme disease.

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